Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4145 | 5' | -58.2 | NC_001664.1 | + | 156067 | 1.13 | 0.001141 |
Target: 5'- aGGAGCAGCCAGUCGAAGCGGGCACGGg -3' miRNA: 3'- -CCUCGUCGGUCAGCUUCGCCCGUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 154418 | 0.69 | 0.662622 |
Target: 5'- cGGGGCGGaUCGGgCG-AG-GGGCGCGGa -3' miRNA: 3'- -CCUCGUC-GGUCaGCuUCgCCCGUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 153583 | 0.66 | 0.860914 |
Target: 5'- gGGGGaCGGgCGGagCGGAG-GGGCGCGa -3' miRNA: 3'- -CCUC-GUCgGUCa-GCUUCgCCCGUGCc -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 153543 | 0.67 | 0.786817 |
Target: 5'- cGGGguGUCGGaCGAcgGGCGGG-GCGGa -3' miRNA: 3'- cCUCguCGGUCaGCU--UCGCCCgUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 152925 | 0.66 | 0.853379 |
Target: 5'- aGAGCAGCgacgaccacCAG-CGcGGCGGuaGCGGa -3' miRNA: 3'- cCUCGUCG---------GUCaGCuUCGCCcgUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 152714 | 0.69 | 0.692293 |
Target: 5'- gGGAGCGGCgAGgcggcCGGAGagccgGGGUACGa -3' miRNA: 3'- -CCUCGUCGgUCa----GCUUCg----CCCGUGCc -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 152407 | 0.67 | 0.795681 |
Target: 5'- cGGcAGgGGUCGGUCGcgccacAGCGGuGCGCGa -3' miRNA: 3'- -CC-UCgUCGGUCAGCu-----UCGCC-CGUGCc -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 150936 | 0.7 | 0.602854 |
Target: 5'- -cAGCGGCgAGUCGGAGUGcGCuCGGg -3' miRNA: 3'- ccUCGUCGgUCAGCUUCGCcCGuGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 4985 | 1.13 | 0.001141 |
Target: 5'- aGGAGCAGCCAGUCGAAGCGGGCACGGg -3' miRNA: 3'- -CCUCGUCGGUCAGCUUCGCCCGUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 3337 | 0.69 | 0.662622 |
Target: 5'- cGGGGCGGaUCGGgCG-AG-GGGCGCGGa -3' miRNA: 3'- -CCUCGUC-GGUCaGCuUCgCCCGUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 2502 | 0.66 | 0.860914 |
Target: 5'- gGGGGaCGGgCGGagCGGAG-GGGCGCGa -3' miRNA: 3'- -CCUC-GUCgGUCa-GCUUCgCCCGUGCc -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 2462 | 0.67 | 0.786817 |
Target: 5'- cGGGguGUCGGaCGAcgGGCGGG-GCGGa -3' miRNA: 3'- cCUCguCGGUCaGCU--UCGCCCgUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 1844 | 0.66 | 0.853379 |
Target: 5'- aGAGCAGCgacgaccacCAG-CGcGGCGGuaGCGGa -3' miRNA: 3'- cCUCGUCG---------GUCaGCuUCGCCcgUGCC- -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 1633 | 0.69 | 0.692293 |
Target: 5'- gGGAGCGGCgAGgcggcCGGAGagccgGGGUACGa -3' miRNA: 3'- -CCUCGUCGgUCa----GCUUCg----CCCGUGCc -5' |
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4145 | 5' | -58.2 | NC_001664.1 | + | 1326 | 0.67 | 0.795681 |
Target: 5'- cGGcAGgGGUCGGUCGcgccacAGCGGuGCGCGa -3' miRNA: 3'- -CC-UCgUCGGUCAGCu-----UCGCC-CGUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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