Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 415 | 5' | -55.9 | AC_000011.1 | + | 17783 | 0.66 | 0.526825 |
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Target: 5'- gGACCCGg--UGCCgccCgCCGGCUCc- -3' miRNA: 3'- aCUGGGUaaaGCGGau-G-GGCCGAGac -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 34785 | 0.66 | 0.51594 |
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Target: 5'- aGAuCCCAUcaUGCCUGa-UGGCUCUGa -3' miRNA: 3'- aCU-GGGUAaaGCGGAUggGCCGAGAC- -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 3824 | 0.68 | 0.412116 |
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Target: 5'- uUGACCCAg--CGCCUgggcgagcugACCCagcagguGGCUCa- -3' miRNA: 3'- -ACUGGGUaaaGCGGA----------UGGG-------CCGAGac -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 6516 | 0.69 | 0.394057 |
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Target: 5'- uUGACCCcgacCuCCUGCCCGGCaUCUu -3' miRNA: 3'- -ACUGGGuaaaGcGGAUGGGCCG-AGAc -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 26581 | 0.69 | 0.394057 |
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Target: 5'- -cACCCGUgagaucaUCGCggGCCCGGCgCUGa -3' miRNA: 3'- acUGGGUAa------AGCGgaUGGGCCGaGAC- -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 21287 | 0.7 | 0.332106 |
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Target: 5'- cUGGCCU---UCGCCUggaACCCGcGCUCg- -3' miRNA: 3'- -ACUGGGuaaAGCGGA---UGGGC-CGAGac -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 20322 | 0.7 | 0.307835 |
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Target: 5'- cGACCCcuacUUCGUCUACUCgGGCUCc- -3' miRNA: 3'- aCUGGGua--AAGCGGAUGGG-CCGAGac -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 35800 | 0.74 | 0.175047 |
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Target: 5'- aGACCCcg-UgGCCUGCCUGGUgCUGg -3' miRNA: 3'- aCUGGGuaaAgCGGAUGGGCCGaGAC- -5' |
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| 415 | 5' | -55.9 | AC_000011.1 | + | 24188 | 1.09 | 0.000498 |
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Target: 5'- cUGACCCAUUUCGCCUACCCGGCUCUGa -3' miRNA: 3'- -ACUGGGUAAAGCGGAUGGGCCGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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