miRNA: 3'- -UGACUCCAAgCGGUgGagGC-UGUACg -5'
miRNA: 3'- -UGACuCCaAGCGGUGGAG------------GCUGUACG- -5'
miRNA: 3'- -UGACuCCaAGCGGUGGAG------------GCUGUACG- -5'
miRNA: 3'- ugACUCCAAGCGGUGGa-GGCUGUa---CG- -5'
miRNA: 3'- -UGACUCCAAGCGGUGGAGGCUGUACG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4150 | 3' | -55 | NC_001664.1 | + | 4973 | 0.66 | 0.94575 |
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Target: 5'- cGCUGGGGUUgGCCAgCg-CGcACGUGa -3' miRNA: 3'- -UGACUCCAAgCGGUgGagGC-UGUACg -5' |
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| 4150 | 3' | -55 | NC_001664.1 | + | 3603 | 0.74 | 0.564086 |
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Target: 5'- cGCUGcGG-UCGCCGCCUCugcggacggaguCGGCGUGUg -3' miRNA: 3'- -UGACuCCaAGCGGUGGAG------------GCUGUACG- -5' |
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| 4150 | 3' | -55 | NC_001664.1 | + | 154684 | 0.74 | 0.564086 |
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Target: 5'- cGCUGcGG-UCGCCGCCUCugcggacggaguCGGCGUGUg -3' miRNA: 3'- -UGACuCCaAGCGGUGGAG------------GCUGUACG- -5' |
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| 4150 | 3' | -55 | NC_001664.1 | + | 102593 | 0.75 | 0.524138 |
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Target: 5'- -gUGAGGUUCGCCGCCgcCUGAUAgaccGCc -3' miRNA: 3'- ugACUCCAAGCGGUGGa-GGCUGUa---CG- -5' |
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| 4150 | 3' | -55 | NC_001664.1 | + | 9721 | 1.11 | 0.00272 |
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Target: 5'- aACUGAGGUUCGCCACCUCCGACAUGCu -3' miRNA: 3'- -UGACUCCAAGCGGUGGAGGCUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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