Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4153 | 3' | -46.8 | NC_001664.1 | + | 90982 | 1.06 | 0.030178 |
Target: 5'- aAAGCAAAAACAAAAUCCAUCCGGGCUu -3' miRNA: 3'- -UUCGUUUUUGUUUUAGGUAGGCCCGA- -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 32899 | 0.69 | 0.994736 |
Target: 5'- cGGUAAAGAUGAGAU-CGUCCGGuGCa -3' miRNA: 3'- uUCGUUUUUGUUUUAgGUAGGCC-CGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 151075 | 0.67 | 0.99947 |
Target: 5'- uGGCGAuAACugu-UCCgGUCCGGGUa -3' miRNA: 3'- uUCGUUuUUGuuuuAGG-UAGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 154072 | 0.67 | 0.99958 |
Target: 5'- gAAGCGGcGACGGAG-CCcgCUGGGUa -3' miRNA: 3'- -UUCGUUuUUGUUUUaGGuaGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 2990 | 0.67 | 0.99958 |
Target: 5'- gAAGCGGcGACGGAG-CCcgCUGGGUa -3' miRNA: 3'- -UUCGUUuUUGUUUUaGGuaGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 154045 | 0.66 | 0.999741 |
Target: 5'- -cGCGGAAguGCGAGA-CCGagCGGGCUc -3' miRNA: 3'- uuCGUUUU--UGUUUUaGGUagGCCCGA- -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 2963 | 0.66 | 0.999741 |
Target: 5'- -cGCGGAAguGCGAGA-CCGagCGGGCUc -3' miRNA: 3'- uuCGUUUU--UGUUUUaGGUagGCCCGA- -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 157027 | 0.66 | 0.999799 |
Target: 5'- uGGGCAAAGACGGAgccguguacGUCCAcCacaugaGGGCg -3' miRNA: 3'- -UUCGUUUUUGUUU---------UAGGUaGg-----CCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 10284 | 0.66 | 0.999846 |
Target: 5'- cAGGUAGGAAgAAGcgcCCAUUCGGGUUg -3' miRNA: 3'- -UUCGUUUUUgUUUua-GGUAGGCCCGA- -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 2490 | 0.76 | 0.871778 |
Target: 5'- cGAGCGAAAccGCGGGuGUCC-UCCGGGCa -3' miRNA: 3'- -UUCGUUUU--UGUUU-UAGGuAGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 5946 | 0.66 | 0.999799 |
Target: 5'- uGGGCAAAGACGGAgccguguacGUCCAcCacaugaGGGCg -3' miRNA: 3'- -UUCGUUUUUGUUU---------UAGGUaGg-----CCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 8383 | 0.67 | 0.99958 |
Target: 5'- -cGUAGAGACGc---CCcgCCGGGCg -3' miRNA: 3'- uuCGUUUUUGUuuuaGGuaGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 40944 | 0.67 | 0.99947 |
Target: 5'- aAAGCGAGAuGCGg---CCAaaacUCCGGGCa -3' miRNA: 3'- -UUCGUUUU-UGUuuuaGGU----AGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 9605 | 0.68 | 0.998749 |
Target: 5'- aAAGUAGAGACGAAGUCCcacacgucGUCCGccgacagcGGCc -3' miRNA: 3'- -UUCGUUUUUGUUUUAGG--------UAGGC--------CCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 153025 | 0.7 | 0.993845 |
Target: 5'- aGAGCAcgaggaaagGAAACAGA--CCGacUCCGGGCg -3' miRNA: 3'- -UUCGU---------UUUUGUUUuaGGU--AGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 1944 | 0.7 | 0.993845 |
Target: 5'- aGAGCAcgaggaaagGAAACAGA--CCGacUCCGGGCg -3' miRNA: 3'- -UUCGU---------UUUUGUUUuaGGU--AGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 26950 | 0.7 | 0.990954 |
Target: 5'- cAGaCAGAAACAu-AUCCAaguaguugcaaucuuUCCGGGCa -3' miRNA: 3'- uUC-GUUUUUGUuuUAGGU---------------AGGCCCGa -5' |
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4153 | 3' | -46.8 | NC_001664.1 | + | 153572 | 0.76 | 0.871778 |
Target: 5'- cGAGCGAAAccGCGGGuGUCC-UCCGGGCa -3' miRNA: 3'- -UUCGUUUU--UGUUU-UAGGuAGGCCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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