Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4156 | 5' | -59.6 | NC_001664.1 | + | 127771 | 1.02 | 0.003306 |
Target: 5'- gAAGCCUCCAGCAGAGCCUCCAGUAGGa -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128035 | 1.07 | 0.001358 |
Target: 5'- aGAGCCUCCAGCAGAGCCUCCAGCAGAg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128155 | 1.05 | 0.001744 |
Target: 5'- aGAGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128119 | 1.05 | 0.001744 |
Target: 5'- aGAGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127975 | 1.05 | 0.00195 |
Target: 5'- aAAGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127843 | 1.05 | 0.00195 |
Target: 5'- aAAGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127627 | 1.05 | 0.00195 |
Target: 5'- aAAGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127903 | 1.04 | 0.002179 |
Target: 5'- aGGGCCUCCAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128083 | 1.02 | 0.003042 |
Target: 5'- aGAGCCUCCAGUAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127687 | 1.02 | 0.003042 |
Target: 5'- aGAGCCUCCAGCAGAGCCUCCAGUAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128202 | 0.94 | 0.010607 |
Target: 5'- aGAGCCUCaAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGgUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127723 | 0.94 | 0.010607 |
Target: 5'- aGAGCCUCaAGCAGAGCCUCCAGCAGGg -3' miRNA: 3'- -UUCGGAGgUCGUCUCGGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127939 | 0.84 | 0.055099 |
Target: 5'- aGAGCCUCCAGCAGGGaUUCUAGCAGGg -3' miRNA: 3'- -UUCGGAGGUCGUCUCgGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128010 | 0.82 | 0.082454 |
Target: 5'- nAGCCUCCAGCAGGGaUUCUAGCAGGg -3' miRNA: 3'- uUCGGAGGUCGUCUCgGAGGUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127818 | 0.8 | 0.110315 |
Target: 5'- nGGCUUCCAGCAGGGCCUCaAGCAa- -3' miRNA: 3'- uUCGGAGGUCGUCUCGGAGgUCGUcu -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127602 | 0.71 | 0.417538 |
Target: 5'- nGGCUUCCAGCAGGGagCUgaGGCAGAu -3' miRNA: 3'- uUCGGAGGUCGUCUCg-GAggUCGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 128058 | 0.71 | 0.426358 |
Target: 5'- nGGCCUCaAGCAaAGCCUCCAGCn-- -3' miRNA: 3'- uUCGGAGgUCGUcUCGGAGGUCGucu -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 127795 | 0.68 | 0.580059 |
Target: 5'- aAAGCCUCCGGUAaAGCCUCaAGCn-- -3' miRNA: 3'- -UUCGGAGGUCGUcUCGGAGgUCGucu -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 85794 | 0.68 | 0.599393 |
Target: 5'- -cGCCUUUcuccucgaucguaGGCAGAGUCUCCAuCGGAa -3' miRNA: 3'- uuCGGAGG-------------UCGUCUCGGAGGUcGUCU- -5' |
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4156 | 5' | -59.6 | NC_001664.1 | + | 24570 | 0.67 | 0.641342 |
Target: 5'- cAAGUC-CUAgaaguugagauGCAGAGCCUCuCAGCAGu -3' miRNA: 3'- -UUCGGaGGU-----------CGUCUCGGAG-GUCGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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