Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4157 | 3' | -48.9 | NC_001664.1 | + | 67911 | 0.66 | 0.998913 |
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Target: 5'- -gGCUGcGAGCGGCcGACuGUUcagAGGGa -3' miRNA: 3'- agUGACuUUUGUCGuCUGuCAG---UCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 134347 | 0.66 | 0.9984 |
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Target: 5'- gUCACUG-AGGCuGUAccuacACAGUUAGGGu -3' miRNA: 3'- -AGUGACuUUUGuCGUc----UGUCAGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 136933 | 0.67 | 0.997695 |
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Target: 5'- aCAUUcGAAuauaGGCAGaACAGUCAGGu -3' miRNA: 3'- aGUGA-CUUuug-UCGUC-UGUCAGUCCc -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 2521 | 0.68 | 0.995502 |
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Target: 5'- aCACgc---GCAGUGGACGG-CGGGGg -3' miRNA: 3'- aGUGacuuuUGUCGUCUGUCaGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 153602 | 0.68 | 0.995502 |
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Target: 5'- aCACgc---GCAGUGGACGG-CGGGGg -3' miRNA: 3'- aGUGacuuuUGUCGUCUGUCaGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 9803 | 0.69 | 0.989288 |
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Target: 5'- cCGCUGu---CGGCGGACGacGUguGGGa -3' miRNA: 3'- aGUGACuuuuGUCGUCUGU--CAguCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 150462 | 0.73 | 0.920444 |
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Target: 5'- aUCGUUGucGAACAGCGGACucgaagaugAGUCGGGGc -3' miRNA: 3'- -AGUGACu-UUUGUCGUCUG---------UCAGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 2480 | 0.77 | 0.746363 |
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Target: 5'- gCGC--GAGACAGCGGACAG-CGGGGu -3' miRNA: 3'- aGUGacUUUUGUCGUCUGUCaGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 153561 | 0.77 | 0.746363 |
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Target: 5'- gCGC--GAGACAGCGGACAG-CGGGGu -3' miRNA: 3'- aGUGacUUUUGUCGUCUGUCaGUCCC- -5' |
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| 4157 | 3' | -48.9 | NC_001664.1 | + | 134575 | 1.13 | 0.007822 |
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Target: 5'- uUCACUGAAAACAGCAGACAGUCAGGGg -3' miRNA: 3'- -AGUGACUUUUGUCGUCUGUCAGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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