Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4160 | 3' | -60.4 | NC_001664.1 | + | 1206 | 1.11 | 0.000858 |
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Target: 5'- gUGGCAGAACAGAGACCCCCCCUGCCCu -3' miRNA: 3'- -ACCGUCUUGUCUCUGGGGGGGACGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 152288 | 1.11 | 0.000858 |
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Target: 5'- gUGGCAGAACAGAGACCCCCCCUGCCCu -3' miRNA: 3'- -ACCGUCUUGUCUCUGGGGGGGACGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 2078 | 0.71 | 0.422459 |
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Target: 5'- cGGCG--GCAGAGGCCgacaugaUUCCUGCCCg -3' miRNA: 3'- aCCGUcuUGUCUCUGGg------GGGGACGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 153160 | 0.71 | 0.422459 |
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Target: 5'- cGGCG--GCAGAGGCCgacaugaUUCCUGCCCg -3' miRNA: 3'- aCCGUcuUGUCUCUGGg------GGGGACGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 4055 | 0.7 | 0.512944 |
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Target: 5'- gGGCGGAACaagGGAGACCgCCCUUuacGCUUu -3' miRNA: 3'- aCCGUCUUG---UCUCUGGgGGGGA---CGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 155137 | 0.7 | 0.512944 |
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Target: 5'- gGGCGGAACaagGGAGACCgCCCUUuacGCUUu -3' miRNA: 3'- aCCGUCUUG---UCUCUGGgGGGGA---CGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 36120 | 0.66 | 0.718921 |
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Target: 5'- aGGUGGAugAgauguuuucuaGAGACgUCCUCUGUCCu -3' miRNA: 3'- aCCGUCUugU-----------CUCUGgGGGGGACGGG- -5' |
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| 4160 | 3' | -60.4 | NC_001664.1 | + | 142422 | 0.66 | 0.738056 |
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Target: 5'- gUGGCGGAACccAGgucuucagucuaAGuuacAUCCCCUUUGCCCu -3' miRNA: 3'- -ACCGUCUUG--UC------------UC----UGGGGGGGACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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