Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4166 | 3' | -61.7 | NC_001664.1 | + | 6433 | 0.66 | 0.720811 |
Target: 5'- aCGC-CGG-GGCCACGCGagguggagaUCgCuuGGGu -3' miRNA: 3'- aGCGuGUCaCCGGUGCGC---------AG-GggCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 157515 | 0.66 | 0.720811 |
Target: 5'- aCGC-CGG-GGCCACGCGagguggagaUCgCuuGGGu -3' miRNA: 3'- aGCGuGUCaCCGGUGCGC---------AG-GggCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 6521 | 0.68 | 0.557065 |
Target: 5'- cCGCACAcgaGCCACG-GUCCCaGGGg -3' miRNA: 3'- aGCGUGUcacCGGUGCgCAGGGgCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 157602 | 0.68 | 0.557065 |
Target: 5'- cCGCACAcgaGCCACG-GUCCCaGGGg -3' miRNA: 3'- aGCGUGUcacCGGUGCgCAGGGgCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 3725 | 0.7 | 0.491796 |
Target: 5'- gCGC-CGGUGGUaccugucguCGCcUCCCCGGGu -3' miRNA: 3'- aGCGuGUCACCGgu-------GCGcAGGGGCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 154807 | 0.7 | 0.491796 |
Target: 5'- gCGC-CGGUGGUaccugucguCGCcUCCCCGGGu -3' miRNA: 3'- aGCGuGUCACCGgu-------GCGcAGGGGCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 6800 | 1.11 | 0.000848 |
Target: 5'- uUCGCACAGUGGCCACGCGUCCCCGGGg -3' miRNA: 3'- -AGCGUGUCACCGGUGCGCAGGGGCCC- -5' |
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4166 | 3' | -61.7 | NC_001664.1 | + | 157882 | 1.11 | 0.000848 |
Target: 5'- uUCGCACAGUGGCCACGCGUCCCCGGGg -3' miRNA: 3'- -AGCGUGUCACCGGUGCGCAGGGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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