Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 417 | 3' | -62.8 | AC_000011.1 | + | 15599 | 0.66 | 0.267462 |
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Target: 5'- uCCCUGGGGCgcccucaaGGGCCgcgugCGGUCg-- -3' miRNA: 3'- -GGGGCCCCGaca-----CCUGGa----GCCGGaug -5' |
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| 417 | 3' | -62.8 | AC_000011.1 | + | 18135 | 0.67 | 0.235807 |
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Target: 5'- cCCCCGGGucGCgcgcGGGCCaCGGCCaccgGCg -3' miRNA: 3'- -GGGGCCC--CGaca-CCUGGaGCCGGa---UG- -5' |
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| 417 | 3' | -62.8 | AC_000011.1 | + | 4071 | 0.68 | 0.183846 |
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Target: 5'- uCCaCCGGGuGCUcaaugaucgagaccgGUGGuCCagGGCCUACc -3' miRNA: 3'- -GG-GGCCC-CGA---------------CACCuGGagCCGGAUG- -5' |
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| 417 | 3' | -62.8 | AC_000011.1 | + | 17728 | 0.68 | 0.177174 |
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Target: 5'- --aCGGGGUgGUGGACCU-GGCCa-- -3' miRNA: 3'- gggGCCCCGaCACCUGGAgCCGGaug -5' |
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| 417 | 3' | -62.8 | AC_000011.1 | + | 16766 | 0.71 | 0.121499 |
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Target: 5'- gCCCGGGGCcaccugcuUGaUGGgccgcaccuugACCUCGGgCUGCa -3' miRNA: 3'- gGGGCCCCG--------AC-ACC-----------UGGAGCCgGAUG- -5' |
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| 417 | 3' | -62.8 | AC_000011.1 | + | 25243 | 1.1 | 0.000092 |
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Target: 5'- aCCCCGGGGCUGUGGACCUCGGCCUACu -3' miRNA: 3'- -GGGGCCCCGACACCUGGAGCCGGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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