Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 417 | 5' | -56.1 | AC_000011.1 | + | 12537 | 0.66 | 0.553301 |
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Target: 5'- -gGUGCCCcagagcGAGGugUACCAgUCCg-- -3' miRNA: 3'- agCACGGG------CUCCugAUGGUaGGGaag -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 31504 | 0.67 | 0.477995 |
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Target: 5'- gUCGcUGCuCCGAGGcGCgGCCGUCCaugUCc -3' miRNA: 3'- -AGC-ACG-GGCUCC-UGaUGGUAGGga-AG- -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 12473 | 0.68 | 0.437283 |
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Target: 5'- aCGUGCCCcggggccagGAGGACUACaccaaCUUCa -3' miRNA: 3'- aGCACGGG---------CUCCUGAUGguaggGAAG- -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 14557 | 0.71 | 0.277396 |
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Target: 5'- gUCaUGCCCGGGGugUAcaccaacgaggcuuuCCAUCCCg-- -3' miRNA: 3'- -AGcACGGGCUCCugAU---------------GGUAGGGaag -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 8371 | 0.72 | 0.234292 |
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Target: 5'- cCGUGCCCcuGGGGugUgaccACCGUCCCc-- -3' miRNA: 3'- aGCACGGG--CUCCugA----UGGUAGGGaag -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 737 | 0.73 | 0.204655 |
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Target: 5'- aUGUGCCCGAGGACg---AUCCCa-- -3' miRNA: 3'- aGCACGGGCUCCUGauggUAGGGaag -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 13376 | 0.75 | 0.150726 |
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Target: 5'- -gGUGCCCGAGG-CcGCCAgUCCUUUCc -3' miRNA: 3'- agCACGGGCUCCuGaUGGU-AGGGAAG- -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 25911 | 0.75 | 0.146522 |
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Target: 5'- uUUGUGCCCccgccAGGACUugUAUCCCUg- -3' miRNA: 3'- -AGCACGGGc----UCCUGAugGUAGGGAag -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 20582 | 0.79 | 0.084635 |
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Target: 5'- aCGUGCCCGAGGGCUACaaggaccgCAUguacUCCUUCu -3' miRNA: 3'- aGCACGGGCUCCUGAUG--------GUA----GGGAAG- -5' |
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| 417 | 5' | -56.1 | AC_000011.1 | + | 25279 | 1.11 | 0.000364 |
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Target: 5'- uUCGUGCCCGAGGACUACCAUCCCUUCg -3' miRNA: 3'- -AGCACGGGCUCCUGAUGGUAGGGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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