Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4170 | 5' | -58.5 | NC_001664.1 | + | 10514 | 0.66 | 0.83882 |
Target: 5'- cUCCGCgagCCUGCg--CCGCgCCUCgUCg -3' miRNA: 3'- uGGGUGa--GGAUGauaGGCG-GGAGgGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 154227 | 0.68 | 0.732036 |
Target: 5'- gAUCCGCUCC-GCgccccUCGCCCgauccgCCCCg -3' miRNA: 3'- -UGGGUGAGGaUGaua--GGCGGGa-----GGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 3145 | 0.68 | 0.732036 |
Target: 5'- gAUCCGCUCC-GCgccccUCGCCCgauccgCCCCg -3' miRNA: 3'- -UGGGUGAGGaUGaua--GGCGGGa-----GGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 455 | 0.68 | 0.702905 |
Target: 5'- cCCCGCUuguuUCUACacuUGUCCGCCC-CUCUa -3' miRNA: 3'- uGGGUGA----GGAUG---AUAGGCGGGaGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 151537 | 0.68 | 0.702905 |
Target: 5'- cCCCGCUuguuUCUACacuUGUCCGCCC-CUCUa -3' miRNA: 3'- uGGGUGA----GGAUG---AUAGGCGGGaGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 4149 | 0.68 | 0.693069 |
Target: 5'- gGCCCGC-CCUc--GUCCucauGCCCUCUCCc -3' miRNA: 3'- -UGGGUGaGGAugaUAGG----CGGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 155231 | 0.68 | 0.693069 |
Target: 5'- gGCCCGC-CCUc--GUCCucauGCCCUCUCCc -3' miRNA: 3'- -UGGGUGaGGAugaUAGG----CGGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 2103 | 0.69 | 0.653322 |
Target: 5'- uGCCCGucgaUCCccCUGUCCGUCCccgggcccgaUCCCCg -3' miRNA: 3'- -UGGGUg---AGGauGAUAGGCGGG----------AGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 153185 | 0.69 | 0.653322 |
Target: 5'- uGCCCGucgaUCCccCUGUCCGUCCccgggcccgaUCCCCg -3' miRNA: 3'- -UGGGUg---AGGauGAUAGGCGGG----------AGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 153307 | 0.7 | 0.592366 |
Target: 5'- cGCCCccccgcaGCUCCUuucGCUAU-CGCCCUgCUCCg -3' miRNA: 3'- -UGGG-------UGAGGA---UGAUAgGCGGGA-GGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 2225 | 0.7 | 0.592366 |
Target: 5'- cGCCCccccgcaGCUCCUuucGCUAU-CGCCCUgCUCCg -3' miRNA: 3'- -UGGG-------UGAGGA---UGAUAgGCGGGA-GGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 3378 | 0.71 | 0.524702 |
Target: 5'- aACCC-CUCCc---AUCC-CCCUCCCCg -3' miRNA: 3'- -UGGGuGAGGaugaUAGGcGGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 154460 | 0.71 | 0.524702 |
Target: 5'- aACCC-CUCCc---AUCC-CCCUCCCCg -3' miRNA: 3'- -UGGGuGAGGaugaUAGGcGGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 156322 | 0.73 | 0.441383 |
Target: 5'- uACUCACUCaCUGuCUGaCCGacauCCCUCCCCg -3' miRNA: 3'- -UGGGUGAG-GAU-GAUaGGC----GGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 5240 | 0.73 | 0.441383 |
Target: 5'- uACUCACUCaCUGuCUGaCCGacauCCCUCCCCg -3' miRNA: 3'- -UGGGUGAG-GAU-GAUaGGC----GGGAGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 153401 | 0.74 | 0.390187 |
Target: 5'- cGCCC-CUCCgc---UCCGCCCgUCCCCc -3' miRNA: 3'- -UGGGuGAGGaugauAGGCGGG-AGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 2319 | 0.74 | 0.390187 |
Target: 5'- cGCCC-CUCCgc---UCCGCCCgUCCCCc -3' miRNA: 3'- -UGGGuGAGGaugauAGGCGGG-AGGGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 153487 | 0.83 | 0.107603 |
Target: 5'- uCCCGCUCCgUugUcUCCGCCCUCuCCCg -3' miRNA: 3'- uGGGUGAGG-AugAuAGGCGGGAG-GGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 2405 | 0.83 | 0.107603 |
Target: 5'- uCCCGCUCCgUugUcUCCGCCCUCuCCCg -3' miRNA: 3'- uGGGUGAGG-AugAuAGGCGGGAG-GGG- -5' |
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4170 | 5' | -58.5 | NC_001664.1 | + | 154157 | 1.12 | 0.001211 |
Target: 5'- gACCCACUCCUACUAUCCGCCCUCCCCg -3' miRNA: 3'- -UGGGUGAGGAUGAUAGGCGGGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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