Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4172 | 5' | -55.6 | NC_001664.1 | + | 14696 | 0.66 | 0.904497 |
Target: 5'- cGCGGgAGaCGGGAACaacaaaccuguugGUAGCGGgCAg -3' miRNA: 3'- uCGCCgUC-GCCUUUGa------------CAUUGCCgGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 9280 | 0.66 | 0.900678 |
Target: 5'- cGGCGGCGGCGuc-GCggugGUGAuCGGCa- -3' miRNA: 3'- -UCGCCGUCGCcuuUGa---CAUU-GCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 108951 | 0.66 | 0.900678 |
Target: 5'- -uUGGCAGCGGAAuacaUGUAugauuugauACGGUCu -3' miRNA: 3'- ucGCCGUCGCCUUug--ACAU---------UGCCGGu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 4832 | 0.67 | 0.857944 |
Target: 5'- cGCGGCAGgcCGuccAAACUGU-ACGGCUAu -3' miRNA: 3'- uCGCCGUC--GCc--UUUGACAuUGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 155913 | 0.67 | 0.857944 |
Target: 5'- cGCGGCAGgcCGuccAAACUGU-ACGGCUAu -3' miRNA: 3'- uCGCCGUC--GCc--UUUGACAuUGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 154542 | 0.67 | 0.850062 |
Target: 5'- gAGCGGgGGUGGGAgggaaGgUGgAugGGCCGg -3' miRNA: 3'- -UCGCCgUCGCCUU-----UgACaUugCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 3461 | 0.67 | 0.850062 |
Target: 5'- gAGCGGgGGUGGGAgggaaGgUGgAugGGCCGg -3' miRNA: 3'- -UCGCCgUCGCCUU-----UgACaUugCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 1629 | 0.68 | 0.833702 |
Target: 5'- gAGCGGgAGCGGcGA----GGCGGCCGg -3' miRNA: 3'- -UCGCCgUCGCCuUUgacaUUGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 152710 | 0.68 | 0.833702 |
Target: 5'- gAGCGGgAGCGGcGA----GGCGGCCGg -3' miRNA: 3'- -UCGCCgUCGCCuUUgacaUUGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 153553 | 0.69 | 0.798809 |
Target: 5'- cAGCGGaCAGCGGGGugUcGgacGACGGgCGg -3' miRNA: 3'- -UCGCC-GUCGCCUUugA-Ca--UUGCCgGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 2472 | 0.69 | 0.798809 |
Target: 5'- cAGCGGaCAGCGGGGugUcGgacGACGGgCGg -3' miRNA: 3'- -UCGCC-GUCGCCUUugA-Ca--UUGCCgGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 1053 | 0.69 | 0.789682 |
Target: 5'- cAGCGGUAGCaacuuGCUGU--CGGCCGa -3' miRNA: 3'- -UCGCCGUCGccuu-UGACAuuGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 152135 | 0.69 | 0.789682 |
Target: 5'- cAGCGGUAGCaacuuGCUGU--CGGCCGa -3' miRNA: 3'- -UCGCCGUCGccuu-UGACAuuGCCGGU- -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 154575 | 0.69 | 0.780409 |
Target: 5'- cGGCGGCGGCGGuugucGCcGUcACGGUa- -3' miRNA: 3'- -UCGCCGUCGCCuu---UGaCAuUGCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 3493 | 0.69 | 0.780409 |
Target: 5'- cGGCGGCGGCGGuugucGCcGUcACGGUa- -3' miRNA: 3'- -UCGCCGUCGCCuu---UGaCAuUGCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 3042 | 0.7 | 0.742061 |
Target: 5'- cGGUGGUGGCGGAGGCggugcgcgagGUGGgGGCg- -3' miRNA: 3'- -UCGCCGUCGCCUUUGa---------CAUUgCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 154124 | 0.7 | 0.742061 |
Target: 5'- cGGUGGUGGCGGAGGCggugcgcgagGUGGgGGCg- -3' miRNA: 3'- -UCGCCGUCGCCUUUGa---------CAUUgCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 152947 | 0.7 | 0.722263 |
Target: 5'- cGGCGGUAGCGGAcgagGACaccgcgaUGACGGCg- -3' miRNA: 3'- -UCGCCGUCGCCU----UUGac-----AUUGCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 1866 | 0.7 | 0.722263 |
Target: 5'- cGGCGGUAGCGGAcgagGACaccgcgaUGACGGCg- -3' miRNA: 3'- -UCGCCGUCGCCU----UUGac-----AUUGCCGgu -5' |
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4172 | 5' | -55.6 | NC_001664.1 | + | 2801 | 0.7 | 0.702149 |
Target: 5'- uGUGuGCGGCGGGcGGCUGUGACuuGCCGa -3' miRNA: 3'- uCGC-CGUCGCCU-UUGACAUUGc-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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