miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4174 5' -59.6 NC_001664.1 + 4007 1.09 0.00135
Target:  5'- uCACACCCCCUGUCCCUAGCUCGCGACg -3'
miRNA:   3'- -GUGUGGGGGACAGGGAUCGAGCGCUG- -5'
4174 5' -59.6 NC_001664.1 + 155089 1.09 0.00135
Target:  5'- uCACACCCCCUGUCCCUAGCUCGCGACg -3'
miRNA:   3'- -GUGUGGGGGACAGGGAUCGAGCGCUG- -5'
4174 5' -59.6 NC_001664.1 + 6333 0.75 0.286776
Target:  5'- -cCACCCCCUGggaCCgUGGCUCGUGuGCg -3'
miRNA:   3'- guGUGGGGGACa--GGgAUCGAGCGC-UG- -5'
4174 5' -59.6 NC_001664.1 + 157415 0.75 0.286776
Target:  5'- -cCACCCCCUGggaCCgUGGCUCGUGuGCg -3'
miRNA:   3'- guGUGGGGGACa--GGgAUCGAGCGC-UG- -5'
4174 5' -59.6 NC_001664.1 + 2295 0.73 0.343033
Target:  5'- gACACCCCgCUGUCCgCUGuCUCGCGcCc -3'
miRNA:   3'- gUGUGGGG-GACAGG-GAUcGAGCGCuG- -5'
4174 5' -59.6 NC_001664.1 + 153377 0.73 0.343033
Target:  5'- gACACCCCgCUGUCCgCUGuCUCGCGcCc -3'
miRNA:   3'- gUGUGGGG-GACAGG-GAUcGAGCGCuG- -5'
4174 5' -59.6 NC_001664.1 + 101315 0.72 0.40682
Target:  5'- aCGCACCCUacaauCUGUCC--AGCUUGCGAUa -3'
miRNA:   3'- -GUGUGGGG-----GACAGGgaUCGAGCGCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.