Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4176 | 5' | -60.6 | NC_001664.1 | + | 4034 | 0.66 | 0.718074 |
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Target: 5'- -gGGCCgGCGcAgACCCUGCCGcggcggcaaaGCCg- -3' miRNA: 3'- gaCCGG-CGCaUgUGGGACGGC----------UGGac -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 155116 | 0.66 | 0.718074 |
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Target: 5'- -gGGCCgGCGcAgACCCUGCCGcggcggcaaaGCCg- -3' miRNA: 3'- gaCCGG-CGCaUgUGGGACGGC----------UGGac -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 37525 | 0.67 | 0.679146 |
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Target: 5'- -cGGCUGCGUcucUCCUGCCGAUuaCUGu -3' miRNA: 3'- gaCCGGCGCAuguGGGACGGCUG--GAC- -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 6134 | 0.68 | 0.600034 |
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Target: 5'- -cGGCCGCcgacgaACACaaCCUGCUGACCg- -3' miRNA: 3'- gaCCGGCGca----UGUG--GGACGGCUGGac -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 157216 | 0.68 | 0.600034 |
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Target: 5'- -cGGCCGCcgacgaACACaaCCUGCUGACCg- -3' miRNA: 3'- gaCCGGCGca----UGUG--GGACGGCUGGac -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 1146 | 0.69 | 0.512797 |
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Target: 5'- gUGG-CGCGaccgAC-CCCUGCCGAgCCUGu -3' miRNA: 3'- gACCgGCGCa---UGuGGGACGGCU-GGAC- -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 152228 | 0.69 | 0.512797 |
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Target: 5'- gUGG-CGCGaccgAC-CCCUGCCGAgCCUGu -3' miRNA: 3'- gACCgGCGCa---UGuGGGACGGCU-GGAC- -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 1239 | 0.72 | 0.373295 |
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Target: 5'- cCUGGCCGCuuucUACGCCCUGUgGAgaCUGc -3' miRNA: 3'- -GACCGGCGc---AUGUGGGACGgCUg-GAC- -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 152321 | 0.72 | 0.373295 |
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Target: 5'- cCUGGCCGCuuucUACGCCCUGUgGAgaCUGc -3' miRNA: 3'- -GACCGGCGc---AUGUGGGACGgCUg-GAC- -5' |
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| 4176 | 5' | -60.6 | NC_001664.1 | + | 9007 | 1.08 | 0.001245 |
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Target: 5'- aCUGGCCGCGUACACCCUGCCGACCUGa -3' miRNA: 3'- -GACCGGCGCAUGUGGGACGGCUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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