Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4181 | 5' | -49.9 | NC_001664.1 | + | 2920 | 0.7 | 0.964269 |
Target: 5'- aGCAGcAGAGUCcGGCGcACgCaCACGCg -3' miRNA: 3'- gCGUU-UUUCAGaCCGUuUGgG-GUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 154390 | 0.66 | 0.997388 |
Target: 5'- gCGCAGGcgcGUCgacgGGCAGuCCCUcggACGCc -3' miRNA: 3'- -GCGUUUuu-CAGa---CCGUUuGGGG---UGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 3308 | 0.66 | 0.997388 |
Target: 5'- gCGCAGGcgcGUCgacgGGCAGuCCCUcggACGCc -3' miRNA: 3'- -GCGUUUuu-CAGa---CCGUUuGGGG---UGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 152511 | 0.67 | 0.994992 |
Target: 5'- uGCGGAGAGggcggaUGGCuu-CCCUuCGCg -3' miRNA: 3'- gCGUUUUUCag----ACCGuuuGGGGuGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 1429 | 0.67 | 0.994992 |
Target: 5'- uGCGGAGAGggcggaUGGCuu-CCCUuCGCg -3' miRNA: 3'- gCGUUUUUCag----ACCGuuuGGGGuGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 119434 | 0.67 | 0.992217 |
Target: 5'- uGUAuAAGAGUCUGcauCAAGCUUCACGUg -3' miRNA: 3'- gCGU-UUUUCAGACc--GUUUGGGGUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 155109 | 0.67 | 0.991059 |
Target: 5'- uCGCGAcgGGcCgGcGCAGACCCUGcCGCg -3' miRNA: 3'- -GCGUUuuUCaGaC-CGUUUGGGGU-GCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 4027 | 0.67 | 0.991059 |
Target: 5'- uCGCGAcgGGcCgGcGCAGACCCUGcCGCg -3' miRNA: 3'- -GCGUUuuUCaGaC-CGUUUGGGGU-GCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 157596 | 0.68 | 0.985018 |
Target: 5'- gGCAAGAucugucGUCUGGCAAACUcguuuuCCACc- -3' miRNA: 3'- gCGUUUUu-----CAGACCGUUUGG------GGUGcg -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 6514 | 0.68 | 0.985018 |
Target: 5'- gGCAAGAucugucGUCUGGCAAACUcguuuuCCACc- -3' miRNA: 3'- gCGUUUUu-----CAGACCGUUUGG------GGUGcg -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 155834 | 0.69 | 0.971891 |
Target: 5'- aCGCAucucGGUCcGGCGcaacgaggcgagacuGGCCCaCGCGCg -3' miRNA: 3'- -GCGUuuu-UCAGaCCGU---------------UUGGG-GUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 4753 | 0.69 | 0.971891 |
Target: 5'- aCGCAucucGGUCcGGCGcaacgaggcgagacuGGCCCaCGCGCg -3' miRNA: 3'- -GCGUuuu-UCAGaCCGU---------------UUGGG-GUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 154001 | 0.7 | 0.964269 |
Target: 5'- aGCAGcAGAGUCcGGCGcACgCaCACGCg -3' miRNA: 3'- gCGUU-UUUCAGaCCGUuUGgG-GUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 16109 | 1.14 | 0.005501 |
Target: 5'- cCGCAAAAAGUCUGGCAAACCCCACGCg -3' miRNA: 3'- -GCGUUUUUCAGACCGUUUGGGGUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 4982 | 0.66 | 0.997388 |
Target: 5'- aGCAGccAGUCgaagcgGGCAcGGgCUCGCGCg -3' miRNA: 3'- gCGUUuuUCAGa-----CCGU-UUgGGGUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 156064 | 0.66 | 0.997388 |
Target: 5'- aGCAGccAGUCgaagcgGGCAcGGgCUCGCGCg -3' miRNA: 3'- gCGUUuuUCAGa-----CCGU-UUgGGGUGCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 142601 | 0.66 | 0.995714 |
Target: 5'- aGCGGAGAauaaagCUGGCAGACCCUc--- -3' miRNA: 3'- gCGUUUUUca----GACCGUUUGGGGugcg -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 5171 | 0.69 | 0.980801 |
Target: 5'- aCGCAucuGGGUCucacgugcgucgcUGGCcAACCCCA-GCg -3' miRNA: 3'- -GCGUuu-UUCAG-------------ACCGuUUGGGGUgCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 156253 | 0.69 | 0.980801 |
Target: 5'- aCGCAucuGGGUCucacgugcgucgcUGGCcAACCCCA-GCg -3' miRNA: 3'- -GCGUuu-UUCAG-------------ACCGuUUGGGGUgCG- -5' |
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4181 | 5' | -49.9 | NC_001664.1 | + | 3143 | 0.73 | 0.881453 |
Target: 5'- gGCGGucgauGUCUGGCGuguugucgugcguGCUCCGCGCg -3' miRNA: 3'- gCGUUuuu--CAGACCGUu------------UGGGGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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