Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
4183 | 5' | -50.6 | NC_001664.1 | + | 1791 | 0.66 | 0.993884 |
Target: 5'- -cGCUCgGGGGacggagaggaagccGGAGGCCUgAGAGGa -3' miRNA: 3'- caUGAGgCCCC--------------CCUUUGGAgUUUUUa -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 152873 | 0.66 | 0.993884 |
Target: 5'- -cGCUCgGGGGacggagaggaagccGGAGGCCUgAGAGGa -3' miRNA: 3'- caUGAGgCCCC--------------CCUUUGGAgUUUUUa -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 3566 | 0.67 | 0.984903 |
Target: 5'- uGUGCUCCGGGGaGGggGa-UgGGAGGg -3' miRNA: 3'- -CAUGAGGCCCC-CCuuUggAgUUUUUa -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 154647 | 0.67 | 0.984903 |
Target: 5'- uGUGCUCCGGGGaGGggGa-UgGGAGGg -3' miRNA: 3'- -CAUGAGGCCCC-CCuuUggAgUUUUUa -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 87951 | 0.68 | 0.978558 |
Target: 5'- gGUGCUCCgaucagGGGGGGAAAaggucaagCGAAGAUa -3' miRNA: 3'- -CAUGAGG------CCCCCCUUUgga-----GUUUUUA- -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 117919 | 0.71 | 0.910281 |
Target: 5'- uGUACUCC-GGGGGAGACUUUc----- -3' miRNA: 3'- -CAUGAGGcCCCCCUUUGGAGuuuuua -5' |
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4183 | 5' | -50.6 | NC_001664.1 | + | 117765 | 1.06 | 0.012663 |
Target: 5'- uGUACUCCGGGGGGAAACCUCAAAAAUg -3' miRNA: 3'- -CAUGAGGCCCCCCUUUGGAGUUUUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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