Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4185 | 3' | -59.4 | NC_001664.1 | + | 2919 | 1.09 | 0.001145 |
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Target: 5'- cAGCAGCAGAGUCCGGCGCACGCACACg -3' miRNA: 3'- -UCGUCGUCUCAGGCCGCGUGCGUGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 154000 | 1.09 | 0.001145 |
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Target: 5'- cAGCAGCAGAGUCCGGCGCACGCACACg -3' miRNA: 3'- -UCGUCGUCUCAGGCCGCGUGCGUGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 6229 | 0.7 | 0.50787 |
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Target: 5'- uGGaCGGCGGu-UCCGGC-CGCGCGCAUg -3' miRNA: 3'- -UC-GUCGUCucAGGCCGcGUGCGUGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 157311 | 0.7 | 0.50787 |
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Target: 5'- uGGaCGGCGGu-UCCGGC-CGCGCGCAUg -3' miRNA: 3'- -UC-GUCGUCucAGGCCGcGUGCGUGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 6365 | 0.68 | 0.636985 |
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Target: 5'- aAGUuGCAGGacacUCCGGCGUGCGUcugACACa -3' miRNA: 3'- -UCGuCGUCUc---AGGCCGCGUGCG---UGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 157446 | 0.68 | 0.636985 |
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Target: 5'- aAGUuGCAGGacacUCCGGCGUGCGUcugACACa -3' miRNA: 3'- -UCGuCGUCUc---AGGCCGCGUGCG---UGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 6162 | 0.73 | 0.364346 |
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Target: 5'- cAGuCGGCAGAGUCUugccgcccucauguGGUGgACGUACACg -3' miRNA: 3'- -UC-GUCGUCUCAGG--------------CCGCgUGCGUGUG- -5' |
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| 4185 | 3' | -59.4 | NC_001664.1 | + | 157244 | 0.73 | 0.364346 |
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Target: 5'- cAGuCGGCAGAGUCUugccgcccucauguGGUGgACGUACACg -3' miRNA: 3'- -UC-GUCGUCUCAGG--------------CCGCgUGCGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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