Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4188 | 3' | -55.6 | NC_001664.1 | + | 6571 | 0.66 | 0.922472 |
Target: 5'- gCCAUGAGaGGACACugGacguacucggccCCgGGUAGAc -3' miRNA: 3'- aGGUACUUgCCUGUGugC------------GG-CCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 143411 | 0.66 | 0.916854 |
Target: 5'- gCgAUGAAUGGAUAuuCACuacauCCGGCGGAg -3' miRNA: 3'- aGgUACUUGCCUGU--GUGc----GGCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 1347 | 0.67 | 0.904908 |
Target: 5'- gUCCcgGcACGucCACAgGCuCGGCAGGg -3' miRNA: 3'- -AGGuaCuUGCcuGUGUgCG-GCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 152428 | 0.67 | 0.904908 |
Target: 5'- gUCCcgGcACGucCACAgGCuCGGCAGGg -3' miRNA: 3'- -AGGuaCuUGCcuGUGUgCG-GCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 145194 | 0.67 | 0.898585 |
Target: 5'- -gUAUGAACGG-UAgACGCCGGaGGAc -3' miRNA: 3'- agGUACUUGCCuGUgUGCGGCCgUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 3396 | 0.67 | 0.885252 |
Target: 5'- cCCcgGAGC--ACACACGCCGacuccguccGCAGAg -3' miRNA: 3'- aGGuaCUUGccUGUGUGCGGC---------CGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 154478 | 0.67 | 0.885252 |
Target: 5'- cCCcgGAGC--ACACACGCCGacuccguccGCAGAg -3' miRNA: 3'- aGGuaCUUGccUGUGUGCGGC---------CGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 13594 | 0.69 | 0.823441 |
Target: 5'- uUCCAUGAACGGAguCucauCGUaaucuuuguaGGCAGGa -3' miRNA: 3'- -AGGUACUUGCCUguGu---GCGg---------CCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 2529 | 0.69 | 0.823441 |
Target: 5'- ----aGaAACGGACACGCGCaguggaCGGCGGGg -3' miRNA: 3'- agguaC-UUGCCUGUGUGCG------GCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 153610 | 0.69 | 0.823441 |
Target: 5'- ----aGaAACGGACACGCGCaguggaCGGCGGGg -3' miRNA: 3'- agguaC-UUGCCUGUGUGCG------GCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 7773 | 0.7 | 0.740034 |
Target: 5'- aCCGUGGgucccagACGGugGCACGUCGcGCGcGAu -3' miRNA: 3'- aGGUACU-------UGCCugUGUGCGGC-CGU-CU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 158855 | 0.7 | 0.740034 |
Target: 5'- aCCGUGGgucccagACGGugGCACGUCGcGCGcGAu -3' miRNA: 3'- aGGUACU-------UGCCugUGUGCGGC-CGU-CU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 157653 | 0.73 | 0.589783 |
Target: 5'- gCCAUGAGaGGACACugGacguacucggccCCGGUAGAc -3' miRNA: 3'- aGGUACUUgCCUGUGugC------------GGCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 153797 | 0.75 | 0.50052 |
Target: 5'- gCCGgc-GCGGACGaggGCGCCGGCGGGa -3' miRNA: 3'- aGGUacuUGCCUGUg--UGCGGCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 2715 | 0.75 | 0.50052 |
Target: 5'- gCCGgc-GCGGACGaggGCGCCGGCGGGa -3' miRNA: 3'- aGGUacuUGCCUGUg--UGCGGCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 154859 | 1.1 | 0.003015 |
Target: 5'- aUCCAUGAACGGACACACGCCGGCAGAa -3' miRNA: 3'- -AGGUACUUGCCUGUGUGCGGCCGUCU- -5' |
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4188 | 3' | -55.6 | NC_001664.1 | + | 3778 | 1.1 | 0.003015 |
Target: 5'- aUCCAUGAACGGACACACGCCGGCAGAa -3' miRNA: 3'- -AGGUACUUGCCUGUGUGCGGCCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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