miRNA: 3'- -AGUACCUUaCUACGC-CGU--CGCGCGcu -5'
miRNA: 3'- agUACCuuACUACG---CCG----UCgCGCG-CU- -5'
miRNA: 3'- -AGUACCuUACUACGCcGUCgCGCGCU- -5'
miRNA: 3'- -AGUACCUUACUACGCCGUCGCGCGCU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4206 | 3' | -55 | NC_001672.1 | + | 8986 | 0.67 | 0.236527 |
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Target: 5'- cUCAUGGGGaGAUGUGcGCAcuGCGUGUa- -3' miRNA: 3'- -AGUACCUUaCUACGC-CGU--CGCGCGcu -5' |
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| 4206 | 3' | -55 | NC_001672.1 | + | 3902 | 0.67 | 0.229599 |
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Target: 5'- -aAUGGggUGAUGCacuGGCcaugGGgGCGCuGAu -3' miRNA: 3'- agUACCuuACUACG---CCG----UCgCGCG-CU- -5' |
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| 4206 | 3' | -55 | NC_001672.1 | + | 10753 | 0.7 | 0.12768 |
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Target: 5'- uUCAUGG-AUGGUGCGuCGG-GUGCGAc -3' miRNA: 3'- -AGUACCuUACUACGCcGUCgCGCGCU- -5' |
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| 4206 | 3' | -55 | NC_001672.1 | + | 10675 | 1.1 | 8.2e-05 |
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Target: 5'- aUCAUGGAAUGAUGCGGCAGCGCGCGAg -3' miRNA: 3'- -AGUACCUUACUACGCCGUCGCGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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