Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4206 | 5' | -56 | NC_001672.1 | + | 565 | 0.66 | 0.255517 |
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Target: 5'- --aCGCACCUGAguUGCugCAUCCu--- -3' miRNA: 3'- ccaGCGUGGGCU--GCGugGUAGGuacu -5' |
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| 4206 | 5' | -56 | NC_001672.1 | + | 7112 | 0.66 | 0.234334 |
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Target: 5'- cGUgGCAUCUGGUGCACaggCCAUGAg -3' miRNA: 3'- cCAgCGUGGGCUGCGUGguaGGUACU- -5' |
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| 4206 | 5' | -56 | NC_001672.1 | + | 9819 | 0.67 | 0.227608 |
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Target: 5'- uGGaCGCACCCugGugGUGCCGUgCCGaGAc -3' miRNA: 3'- -CCaGCGUGGG--CugCGUGGUA-GGUaCU- -5' |
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| 4206 | 5' | -56 | NC_001672.1 | + | 10712 | 0.71 | 0.113132 |
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Target: 5'- -cUCGCGCgCUGcCGCAUCAUuCCAUGAu -3' miRNA: 3'- ccAGCGUG-GGCuGCGUGGUA-GGUACU- -5' |
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| 4206 | 5' | -56 | NC_001672.1 | + | 3897 | 0.73 | 0.074915 |
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Target: 5'- aGGcUCGCACCCGGCaGCcCCAauUCCAgGAc -3' miRNA: 3'- -CC-AGCGUGGGCUG-CGuGGU--AGGUaCU- -5' |
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| 4206 | 5' | -56 | NC_001672.1 | + | 10716 | 1.11 | 0.000067 |
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Target: 5'- uGGUCGCACCCGACGCACCAUCCAUGAa -3' miRNA: 3'- -CCAGCGUGGGCUGCGUGGUAGGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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