miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
421 5' -54.6 AC_000011.1 + 24606 0.67 0.618832
Target:  5'- aCGUGCUGCacaccaccCUGCGcggGGAgGCCCGGc -3'
miRNA:   3'- gGCAUGAUGaa------GGCGC---UCUgCGGGUC- -5'
421 5' -54.6 AC_000011.1 + 24967 0.7 0.416938
Target:  5'- uUCGUGCcGCUgaccuUCCGCGAG-UGCCCc- -3'
miRNA:   3'- -GGCAUGaUGA-----AGGCGCUCuGCGGGuc -5'
421 5' -54.6 AC_000011.1 + 7516 0.72 0.336127
Target:  5'- cCCGUGCUGCU----CGAG-CGCCCAGu -3'
miRNA:   3'- -GGCAUGAUGAaggcGCUCuGCGGGUC- -5'
421 5' -54.6 AC_000011.1 + 15570 0.74 0.258419
Target:  5'- cCCGUGCgcgugcgcgggcACUUCCGCGcucccugGGGCGCCCu- -3'
miRNA:   3'- -GGCAUGa-----------UGAAGGCGC-------UCUGCGGGuc -5'
421 5' -54.6 AC_000011.1 + 21937 0.74 0.247101
Target:  5'- gUCGguccACaGCUUCCGCGcaguugcaGGGCGCCCAGc -3'
miRNA:   3'- -GGCa---UGaUGAAGGCGC--------UCUGCGGGUC- -5'
421 5' -54.6 AC_000011.1 + 26676 1.12 0.000505
Target:  5'- aCCGUACUACUUCCGCGAGACGCCCAGg -3'
miRNA:   3'- -GGCAUGAUGAAGGCGCUCUGCGGGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.