Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 422 | 3' | -52 | AC_000011.1 | + | 7079 | 0.66 | 0.750293 |
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Target: 5'- uGGUUGAcgG-CCUu--GUaGGCGCAGCa -3' miRNA: 3'- -CUAACUuaCaGGGuacCA-CCGCGUCG- -5' |
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| 422 | 3' | -52 | AC_000011.1 | + | 35291 | 0.66 | 0.727976 |
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Target: 5'- ---aGAAUG-CCgGUGGagGGCGCucuGCg -3' miRNA: 3'- cuaaCUUACaGGgUACCa-CCGCGu--CG- -5' |
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| 422 | 3' | -52 | AC_000011.1 | + | 4086 | 0.67 | 0.716648 |
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Target: 5'- --aUGAGcccGUCCCGggGGUGGagGUAGCu -3' miRNA: 3'- cuaACUUa--CAGGGUa-CCACCg-CGUCG- -5' |
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| 422 | 3' | -52 | AC_000011.1 | + | 35029 | 0.68 | 0.647167 |
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Target: 5'- ----aGAUGUUCCAcGGUGGCuucCAGCa -3' miRNA: 3'- cuaacUUACAGGGUaCCACCGc--GUCG- -5' |
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| 422 | 3' | -52 | AC_000011.1 | + | 11430 | 0.75 | 0.289511 |
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Target: 5'- ------cUGUUCC-UGGUGGUGCAGCa -3' miRNA: 3'- cuaacuuACAGGGuACCACCGCGUCG- -5' |
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| 422 | 3' | -52 | AC_000011.1 | + | 27091 | 1.12 | 0.000645 |
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Target: 5'- cGAUUGAAUGUCCCAUGGUGGCGCAGCu -3' miRNA: 3'- -CUAACUUACAGGGUACCACCGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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