Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 424 | 5' | -60.2 | AC_000011.1 | + | 14253 | 0.66 | 0.380581 |
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Target: 5'- gCCGCCCCaccGCGaG-CGCUCGUCa-- -3' miRNA: 3'- gGGCGGGGau-UGC-CaGCGGGCAGaga -5' |
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| 424 | 5' | -60.2 | AC_000011.1 | + | 9445 | 0.68 | 0.292773 |
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Target: 5'- gCgGCCCCgcgagGACGGgCGCgCCGUCa-- -3' miRNA: 3'- gGgCGGGGa----UUGCCaGCG-GGCAGaga -5' |
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| 424 | 5' | -60.2 | AC_000011.1 | + | 15689 | 0.69 | 0.245618 |
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Target: 5'- cCCCGCCgCCg------CGCCCGUCUCc -3' miRNA: 3'- -GGGCGG-GGauugccaGCGGGCAGAGa -5' |
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| 424 | 5' | -60.2 | AC_000011.1 | + | 8528 | 0.69 | 0.245618 |
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Target: 5'- gCCGCCCCUGccgccCGG-CGCgCGUcCUCg -3' miRNA: 3'- gGGCGGGGAUu----GCCaGCGgGCA-GAGa -5' |
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| 424 | 5' | -60.2 | AC_000011.1 | + | 153 | 0.87 | 0.011636 |
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Target: 5'- cCCCGCCCCUAACGGUCGCUCauccCUCg -3' miRNA: 3'- -GGGCGGGGAUUGCCAGCGGGca--GAGa -5' |
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| 424 | 5' | -60.2 | AC_000011.1 | + | 36367 | 1.08 | 0.000262 |
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Target: 5'- cCCCGCCCCUAACGGUCGCCCGUCUCUc -3' miRNA: 3'- -GGGCGGGGAUUGCCAGCGGGCAGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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