miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4250 5' -58.9 NC_001702.1 + 6402 1.08 0.000032
Target:  5'- aAGACUCUACCGAUCCACGGGGGCCGAc -3'
miRNA:   3'- -UCUGAGAUGGCUAGGUGCCCCCGGCU- -5'
4250 5' -58.9 NC_001702.1 + 752 0.67 0.095016
Target:  5'- -uGCUCUGCCGAguggCCAacuuucgguguaGGGuGGCCGc -3'
miRNA:   3'- ucUGAGAUGGCUa---GGUg-----------CCC-CCGGCu -5'
4250 5' -58.9 NC_001702.1 + 2172 0.67 0.098725
Target:  5'- uGGGCUagggaUUGCCcaaagaagCCACGGGGGCCc- -3'
miRNA:   3'- -UCUGA-----GAUGGcua-----GGUGCCCCCGGcu -5'
4250 5' -58.9 NC_001702.1 + 3864 0.67 0.108604
Target:  5'- uGGCUgCUACC-AUCCGuaggcaaGGGGGCCa- -3'
miRNA:   3'- uCUGA-GAUGGcUAGGUg------CCCCCGGcu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.