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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4251 | 3' | -56.2 | NC_001702.1 | + | 5431 | 0.71 | 0.07307 |
Target: 5'- gGGCaCCCCCGCCccuugucaaUUUUCAugAuCCUGa -3' miRNA: 3'- -CCGaGGGGGCGG---------AAGAGUugUuGGAC- -5' |
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4251 | 3' | -56.2 | NC_001702.1 | + | 6599 | 1.11 | 3.2e-05 |
Target: 5'- gGGCUCCCCCGCCUUCUCAACAACCUGg -3' miRNA: 3'- -CCGAGGGGGCGGAAGAGUUGUUGGAC- -5' |
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4251 | 3' | -56.2 | NC_001702.1 | + | 6671 | 0.66 | 0.164335 |
Target: 5'- aGGCUCCUUUuaCUaggUUUAGCAGCCUGu -3' miRNA: 3'- -CCGAGGGGGcgGAa--GAGUUGUUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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