Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4254 | 3' | -61 | NC_001710.1 | + | 3418 | 0.66 | 0.091124 |
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Target: 5'- uCGCCaCACCCguggGGGCCCuuaAUCCCAg -3' miRNA: 3'- uGUGGaGUGGG----UCCGGGuccUAGGGU- -5' |
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| 4254 | 3' | -61 | NC_001710.1 | + | 5247 | 0.66 | 0.091124 |
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Target: 5'- cACGCCUCAgCCGguGGUgCAGGuUCCUc -3' miRNA: 3'- -UGUGGAGUgGGU--CCGgGUCCuAGGGu -5' |
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| 4254 | 3' | -61 | NC_001710.1 | + | 6687 | 0.67 | 0.075292 |
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Target: 5'- uACACCUUGCCUcaucaacugAGGCUCAGGAauguggcaCCCu -3' miRNA: 3'- -UGUGGAGUGGG---------UCCGGGUCCUa-------GGGu -5' |
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| 4254 | 3' | -61 | NC_001710.1 | + | 1816 | 0.68 | 0.056393 |
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Target: 5'- -uGCCUCgGCCCugguuGCCCAGGggCCCc -3' miRNA: 3'- ugUGGAG-UGGGuc---CGGGUCCuaGGGu -5' |
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| 4254 | 3' | -61 | NC_001710.1 | + | 7927 | 0.69 | 0.051183 |
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Target: 5'- cCGCCUUggcuACCC-GGCCUGGGucUCCCAa -3' miRNA: 3'- uGUGGAG----UGGGuCCGGGUCCu-AGGGU- -5' |
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| 4254 | 3' | -61 | NC_001710.1 | + | 879 | 1.07 | 0.000021 |
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Target: 5'- uACACCUCACCCAGGCCCAGGAUCCCAg -3' miRNA: 3'- -UGUGGAGUGGGUCCGGGUCCUAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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