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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4255 | 3' | -54.7 | NC_001710.1 | + | 1566 | 1.08 | 0.000087 |
Target: 5'- uGAUCCAAGUGCGGCUAUGGUGCAGGUg -3' miRNA: 3'- -CUAGGUUCACGCCGAUACCACGUCCA- -5' |
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4255 | 3' | -54.7 | NC_001710.1 | + | 4144 | 0.7 | 0.117291 |
Target: 5'- aGUCCGGGaGCuGGCgcgugGGUGCGGGg -3' miRNA: 3'- cUAGGUUCaCG-CCGaua--CCACGUCCa -5' |
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4255 | 3' | -54.7 | NC_001710.1 | + | 2246 | 0.68 | 0.147285 |
Target: 5'- uGAUCUugcUGCuGCUAUGGUGguGGg -3' miRNA: 3'- -CUAGGuucACGcCGAUACCACguCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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