Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4256 | 3' | -51 | NC_001710.1 | + | 42 | 0.66 | 0.461475 |
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Target: 5'- aGUGCCGggGGGG-GGGGAUC-AACUc -3' miRNA: 3'- gCGUGGCuaCCUUaCCCUUGGaUUGG- -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 4702 | 0.66 | 0.457985 |
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Target: 5'- gGCGgUGGaagcUGGAGUGaccugguacggaauGGAACCUGACUu -3' miRNA: 3'- gCGUgGCU----ACCUUAC--------------CCUUGGAUUGG- -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 782 | 0.66 | 0.427238 |
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Target: 5'- gGCccaACUGGUGGGGgagcUGGGuAGCCUAuacggGCCc -3' miRNA: 3'- gCG---UGGCUACCUU----ACCC-UUGGAU-----UGG- -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 7666 | 0.67 | 0.405268 |
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Target: 5'- uGCugCcAUGGGcUGGGGAUCUAAg- -3' miRNA: 3'- gCGugGcUACCUuACCCUUGGAUUgg -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 5300 | 0.7 | 0.264755 |
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Target: 5'- --gGCCGAUGGucuaccgGaGGAACCUGcACCa -3' miRNA: 3'- gcgUGGCUACCuua----C-CCUUGGAU-UGG- -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 3031 | 0.72 | 0.183033 |
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Target: 5'- aGCGCCuguUGGAAUGGGAcgcaGCUcuugAACCu -3' miRNA: 3'- gCGUGGcu-ACCUUACCCU----UGGa---UUGG- -5' |
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| 4256 | 3' | -51 | NC_001710.1 | + | 1715 | 1.14 | 0.00011 |
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Target: 5'- cCGCACCGAUGGAAUGGGAACCUAACCg -3' miRNA: 3'- -GCGUGGCUACCUUACCCUUGGAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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