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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4263 | 5' | -52.3 | NC_001710.1 | + | 295 | 0.67 | 0.286841 |
Target: 5'- gCCCGUUaCCCaccUGGGCA--AACGACGc -3' miRNA: 3'- aGGGCGA-GGGc--AUCCGUauUUGUUGC- -5' |
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4263 | 5' | -52.3 | NC_001710.1 | + | 428 | 0.73 | 0.123547 |
Target: 5'- gUCCCGCccuUCCCgGUGGGCcgGGaaauGCAugGg -3' miRNA: 3'- -AGGGCG---AGGG-CAUCCGuaUU----UGUugC- -5' |
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4263 | 5' | -52.3 | NC_001710.1 | + | 3854 | 1.11 | 0.000124 |
Target: 5'- uUCCCGCUCCCGUAGGCAUAAACAACGg -3' miRNA: 3'- -AGGGCGAGGGCAUCCGUAUUUGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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