Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4266 | 5' | -53.3 | NC_001710.1 | + | 2949 | 0.66 | 0.306711 |
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Target: 5'- --aACCGUccGGCUggUCUCCaaGAUGUguGCg -3' miRNA: 3'- uugUGGCG--CCGA--AGAGG--CUAUAguCG- -5' |
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| 4266 | 5' | -53.3 | NC_001710.1 | + | 2329 | 0.66 | 0.306711 |
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Target: 5'- -cCACgGCGGCUUCcacagCCGg---CAGCc -3' miRNA: 3'- uuGUGgCGCCGAAGa----GGCuauaGUCG- -5' |
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| 4266 | 5' | -53.3 | NC_001710.1 | + | 7932 | 0.66 | 0.272758 |
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Target: 5'- cAACACCGCcuuGGCUacccggccugggUCUCCcaaGAUGagcuuuUCAGCu -3' miRNA: 3'- -UUGUGGCG---CCGA------------AGAGG---CUAU------AGUCG- -5' |
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| 4266 | 5' | -53.3 | NC_001710.1 | + | 5603 | 0.68 | 0.201097 |
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Target: 5'- uGGCAgCCGCGGCUUCcuacCUGAUGggguUgGGCg -3' miRNA: 3'- -UUGU-GGCGCCGAAGa---GGCUAU----AgUCG- -5' |
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| 4266 | 5' | -53.3 | NC_001710.1 | + | 4784 | 0.69 | 0.188889 |
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Target: 5'- uACACCGCaGCcgUCgcggCUGAUAUCGGa -3' miRNA: 3'- uUGUGGCGcCGa-AGa---GGCUAUAGUCg -5' |
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| 4266 | 5' | -53.3 | NC_001710.1 | + | 4836 | 1.09 | 0.000114 |
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Target: 5'- gAACACCGCGGCUUCUCCGAUAUCAGCc -3' miRNA: 3'- -UUGUGGCGCCGAAGAGGCUAUAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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