miRNA: 3'- -UGGACgugg-UGGCCGACUCCGcaCCG- -5'
miRNA: 3'- uGGA-CgUGGUGGCCGACuCC-------GCACCG- -5'
miRNA: 3'- uGGACGuGGUGGc--CGACUCCGCaCCG- -5'
miRNA: 3'- -UGGAcGUGGUGg---CCG-ACU--CCGCACCG- -5'
miRNA: 3'- -UGGACGUGGUGGCCGACUCCGCACCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4268 | 5' | -61.3 | NC_001710.1 | + | 2209 | 0.66 | 0.081368 |
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Target: 5'- uACCUGaugaaGCUGGCUGAGGCacGGUu -3' miRNA: 3'- -UGGACgugg-UGGCCGACUCCGcaCCG- -5' |
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| 4268 | 5' | -61.3 | NC_001710.1 | + | 2288 | 0.7 | 0.038768 |
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Target: 5'- uCCUaGgGCUGCCGGCUGuGGaagccgcCGUGGCa -3' miRNA: 3'- uGGA-CgUGGUGGCCGACuCC-------GCACCG- -5' |
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| 4268 | 5' | -61.3 | NC_001710.1 | + | 4992 | 0.71 | 0.035287 |
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Target: 5'- uCCUGUcCCACUccuGCUGAGGUGgGGCa -3' miRNA: 3'- uGGACGuGGUGGc--CGACUCCGCaCCG- -5' |
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| 4268 | 5' | -61.3 | NC_001710.1 | + | 7460 | 0.71 | 0.032008 |
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Target: 5'- gGCCU-CACCACgggaGGCuUGGccGGCGUGGCc -3' miRNA: 3'- -UGGAcGUGGUGg---CCG-ACU--CCGCACCG- -5' |
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| 4268 | 5' | -61.3 | NC_001710.1 | + | 5280 | 1.09 | 0.000014 |
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Target: 5'- aACCUGCACCACCGGCUGAGGCGUGGCc -3' miRNA: 3'- -UGGACGUGGUGGCCGACUCCGCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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