Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4269 | 3' | -53.9 | NC_001710.1 | + | 5595 | 0.66 | 0.30008 |
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Target: 5'- cCCGcCGUUGGCAgccGCGgCUUCCU-ACCu -3' miRNA: 3'- cGGC-GCAACUGU---UGUgGGAGGGuUGG- -5' |
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| 4269 | 3' | -53.9 | NC_001710.1 | + | 7117 | 0.67 | 0.259686 |
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Target: 5'- aGCCaCGUUGAaGACACUUUCCUccCCu -3' miRNA: 3'- -CGGcGCAACUgUUGUGGGAGGGuuGG- -5' |
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| 4269 | 3' | -53.9 | NC_001710.1 | + | 490 | 0.69 | 0.192285 |
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Target: 5'- cGCCGCGgaGcuGgGugGCCCcaugcauuUCCCGGCCc -3' miRNA: 3'- -CGGCGCaaC--UgUugUGGG--------AGGGUUGG- -5' |
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| 4269 | 3' | -53.9 | NC_001710.1 | + | 7730 | 0.69 | 0.174737 |
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Target: 5'- cCCGCGggggUGGCuaaguccuuaaccGACACCUUagaucCCCAGCCc -3' miRNA: 3'- cGGCGCa---ACUG-------------UUGUGGGA-----GGGUUGG- -5' |
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| 4269 | 3' | -53.9 | NC_001710.1 | + | 893 | 0.75 | 0.066706 |
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Target: 5'- aGUCGCGUUGACGcgccgggucUACCCggugCCUAACCu -3' miRNA: 3'- -CGGCGCAACUGUu--------GUGGGa---GGGUUGG- -5' |
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| 4269 | 3' | -53.9 | NC_001710.1 | + | 5834 | 1.14 | 0.000047 |
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Target: 5'- cGCCGCGUUGACAACACCCUCCCAACCu -3' miRNA: 3'- -CGGCGCAACUGUUGUGGGAGGGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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