Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4274 | 5' | -51.9 | NC_001710.1 | + | 4132 | 0.66 | 0.35021 |
Target: 5'- aGGcauUGGgaGAGUCCgggAGCUGGCGCg -3' miRNA: 3'- cCCc--ACCgaUUCAGGaa-UUGGCUGUG- -5' |
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4274 | 5' | -51.9 | NC_001710.1 | + | 7719 | 0.68 | 0.294897 |
Target: 5'- cGGGGaagaUGGCc--GUCCaUGACCGGCu- -3' miRNA: 3'- -CCCC----ACCGauuCAGGaAUUGGCUGug -5' |
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4274 | 5' | -51.9 | NC_001710.1 | + | 2807 | 0.68 | 0.286367 |
Target: 5'- uGGuGGUGGUUGccuuGGUCCUucucuuUGGCCuguucGACGCg -3' miRNA: 3'- -CC-CCACCGAU----UCAGGA------AUUGG-----CUGUG- -5' |
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4274 | 5' | -51.9 | NC_001710.1 | + | 5209 | 0.72 | 0.13549 |
Target: 5'- gGGGGUGGCgccccCCUUuAUCGGCAUg -3' miRNA: 3'- -CCCCACCGauucaGGAAuUGGCUGUG- -5' |
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4274 | 5' | -51.9 | NC_001710.1 | + | 7725 | 1.11 | 0.000123 |
Target: 5'- gGGGGUGGCUAAGUCCUUAACCGACACc -3' miRNA: 3'- -CCCCACCGAUUCAGGAAUUGGCUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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