Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4280 | 5' | -59.2 | NC_001710.1 | + | 6325 | 0.66 | 0.134285 |
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Target: 5'- aCgCAGCCACagaGgCAGCGACU-CUCa -3' miRNA: 3'- gGgGUUGGUGg--CgGUCGCUGAgGAGa -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 4182 | 0.67 | 0.10145 |
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Target: 5'- aCCCCGcacCCACgCGCCAGCucccgGACUCUc-- -3' miRNA: 3'- -GGGGUu--GGUG-GCGGUCG-----CUGAGGaga -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 6828 | 0.68 | 0.092297 |
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Target: 5'- --gCAGCaGCCGCCGGCGGCagaucggCCUCa -3' miRNA: 3'- gggGUUGgUGGCGGUCGCUGa------GGAGa -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 5640 | 0.68 | 0.091426 |
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Target: 5'- aCCCCAucagguaggaagccGCgGCUGCCaacGGCGgGCUCCUUg -3' miRNA: 3'- -GGGGU--------------UGgUGGCGG---UCGC-UGAGGAGa -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 600 | 0.7 | 0.062963 |
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Target: 5'- gCCCCGGCCACCcacGCUugucgAGCGAaugggcaauauUUCCUCa -3' miRNA: 3'- -GGGGUUGGUGG---CGG-----UCGCU-----------GAGGAGa -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 6944 | 0.71 | 0.051898 |
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Target: 5'- cCCCCGuacuCCAUCGCCAGC-ACUuaUCUCg -3' miRNA: 3'- -GGGGUu---GGUGGCGGUCGcUGA--GGAGa -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 6795 | 0.71 | 0.048648 |
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Target: 5'- -gCCGAUCuGCCGCCGGCGGCUgCUg- -3' miRNA: 3'- ggGGUUGG-UGGCGGUCGCUGAgGAga -5' |
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| 4280 | 5' | -59.2 | NC_001710.1 | + | 9045 | 1.1 | 0.000026 |
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Target: 5'- aCCCCAACCACCGCCAGCGACUCCUCUg -3' miRNA: 3'- -GGGGUUGGUGGCGGUCGCUGAGGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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