Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4287 | 5' | -52.8 | NC_001710.1 | + | 858 | 0.66 | 0.305202 |
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Target: 5'- cUGGGUGaGGUGUAcucggguguccuaacGGUGGGAGucgcguugacGCGCCg -3' miRNA: 3'- -ACUCACgUUACGU---------------CUACCCUU----------CGCGG- -5' |
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| 4287 | 5' | -52.8 | NC_001710.1 | + | 6116 | 0.66 | 0.301656 |
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Target: 5'- -cAGgGCAaccacuGUGCGGGUGaGGcucAGGCGCCg -3' miRNA: 3'- acUCaCGU------UACGUCUAC-CC---UUCGCGG- -5' |
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| 4287 | 5' | -52.8 | NC_001710.1 | + | 2915 | 0.69 | 0.190847 |
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Target: 5'- uUGAGUGCugugugaugGCGGGUGaGAAG-GCCa -3' miRNA: 3'- -ACUCACGuua------CGUCUACcCUUCgCGG- -5' |
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| 4287 | 5' | -52.8 | NC_001710.1 | + | 7904 | 0.69 | 0.190847 |
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Target: 5'- cGGGUaGCcaagGCGGuguUGGGggGgGCCu -3' miRNA: 3'- aCUCA-CGuua-CGUCu--ACCCuuCgCGG- -5' |
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| 4287 | 5' | -52.8 | NC_001710.1 | + | 5265 | 0.71 | 0.142496 |
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Target: 5'- cUGAG-GCGuggccuggucuccAUGCcGAUaaaGGGggGCGCCa -3' miRNA: 3'- -ACUCaCGU-------------UACGuCUA---CCCuuCGCGG- -5' |
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| 4287 | 5' | -52.8 | NC_001710.1 | + | 8444 | 1.13 | 0.000056 |
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Target: 5'- cUGAGUGCAAUGCAGAUGGGAAGCGCCa -3' miRNA: 3'- -ACUCACGUUACGUCUACCCUUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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