miRNA: 3'- -UGa-GUGGC-GUC-AGGGCGUG-GCa----CGCU- -5'
miRNA: 3'- -UGa-GUGGC-GUC-AGGGCGUG-GCa----CGCU- -5'
miRNA: 3'- -UGAGUGGCGUCAGGGCGUGGCACGCU- -5'
miRNA: 3'- -UGAGUGGCGUCAGGGCGUGGCACGCU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4289 | 3' | -59.8 | NC_001716.2 | + | 148553 | 0.68 | 0.651308 |
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Target: 5'- cGCggCGCCGaCAGuUCCCGCGCgCGcagggGCGGg -3' miRNA: 3'- -UGa-GUGGC-GUC-AGGGCGUG-GCa----CGCU- -5' |
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| 4289 | 3' | -59.8 | NC_001716.2 | + | 5650 | 0.68 | 0.651308 |
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Target: 5'- cGCggCGCCGaCAGuUCCCGCGCgCGcagggGCGGg -3' miRNA: 3'- -UGa-GUGGC-GUC-AGGGCGUG-GCa----CGCU- -5' |
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| 4289 | 3' | -59.8 | NC_001716.2 | + | 146002 | 1.08 | 0.00154 |
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Target: 5'- cACUCACCGCAGUCCCGCACCGUGCGAg -3' miRNA: 3'- -UGAGUGGCGUCAGGGCGUGGCACGCU- -5' |
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| 4289 | 3' | -59.8 | NC_001716.2 | + | 3099 | 1.08 | 0.00154 |
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Target: 5'- cACUCACCGCAGUCCCGCACCGUGCGAg -3' miRNA: 3'- -UGAGUGGCGUCAGGGCGUGGCACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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