Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4293 | 5' | -42.5 | NC_001716.2 | + | 113636 | 0.66 | 1 |
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Target: 5'- -cCAUACUAACAUAGauauguuucauUAUAGAGAUAAa -3' miRNA: 3'- uuGUGUGGUUGUAUU-----------AUGUUUCUGUU- -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 47017 | 0.66 | 1 |
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Target: 5'- aAAUACACUgaAACGUuuuGUGCAAAGugGc -3' miRNA: 3'- -UUGUGUGG--UUGUAu--UAUGUUUCugUu -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 122632 | 0.68 | 0.999998 |
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Target: 5'- aAACACAgCUAACGag--ACAGAGACAGc -3' miRNA: 3'- -UUGUGU-GGUUGUauuaUGUUUCUGUU- -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 91575 | 0.7 | 0.999943 |
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Target: 5'- --aAUAUCAACGauAUGCAAAGACAAa -3' miRNA: 3'- uugUGUGGUUGUauUAUGUUUCUGUU- -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 148636 | 0.7 | 0.999942 |
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Target: 5'- cGCACAgCCAagagagcGCGgAGUGCAGAGGCAAu -3' miRNA: 3'- uUGUGU-GGU-------UGUaUUAUGUUUCUGUU- -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 5733 | 0.7 | 0.999942 |
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Target: 5'- cGCACAgCCAagagagcGCGgAGUGCAGAGGCAAu -3' miRNA: 3'- uUGUGU-GGU-------UGUaUUAUGUUUCUGUU- -5' |
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| 4293 | 5' | -42.5 | NC_001716.2 | + | 37051 | 1.05 | 0.087266 |
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Target: 5'- cAACACACCAACAUAAUACAAAGACAAu -3' miRNA: 3'- -UUGUGUGGUUGUAUUAUGUUUCUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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