Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4307 | 3' | -50.2 | NC_001716.2 | + | 134813 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135023 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135232 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135337 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135442 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135547 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135652 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135757 | 0.66 | 0.994541 |
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Target: 5'- gUCAGUGGGUUauguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAa-----AugCCCA---AGaGugGG- -5' |
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| 4307 | 3' | -50.2 | NC_001716.2 | + | 135127 | 0.69 | 0.972137 |
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Target: 5'- gUCAGUGGGUUUguggUugGGGUcuuUC-CugCCu -3' miRNA: 3'- -AGUCGUCCAAA----AugCCCA---AGaGugGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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