Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4321 | 3' | -55.7 | NC_001720.1 | + | 960 | 0.67 | 0.560128 |
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Target: 5'- cUCGcGGUCgGGCGCUaGUUCCCACAGa-- -3' miRNA: 3'- -GGC-CUAG-UCGCGA-CAGGGGUGUUguc -5' |
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| 4321 | 3' | -55.7 | NC_001720.1 | + | 12007 | 0.67 | 0.549247 |
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Target: 5'- gCCGcGAUCGccgaacgucgccGCGCcG-CCCUACAACGGa -3' miRNA: 3'- -GGC-CUAGU------------CGCGaCaGGGGUGUUGUC- -5' |
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| 4321 | 3' | -55.7 | NC_001720.1 | + | 28930 | 0.68 | 0.538433 |
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Target: 5'- uCgGGGUCAGCGgUGagCCCGCcgcCAGa -3' miRNA: 3'- -GgCCUAGUCGCgACagGGGUGuu-GUC- -5' |
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| 4321 | 3' | -55.7 | NC_001720.1 | + | 29086 | 0.68 | 0.527693 |
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Target: 5'- cCCGacGAUCAGCGCUucucCCCCGCuaaccuacaccAACGGg -3' miRNA: 3'- -GGC--CUAGUCGCGAca--GGGGUG-----------UUGUC- -5' |
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| 4321 | 3' | -55.7 | NC_001720.1 | + | 12325 | 0.72 | 0.297093 |
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Target: 5'- cCCGGcgCgGGCGCgacaCCCACGGCAGg -3' miRNA: 3'- -GGCCuaG-UCGCGacagGGGUGUUGUC- -5' |
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| 4321 | 3' | -55.7 | NC_001720.1 | + | 2352 | 1.1 | 0.000602 |
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Target: 5'- aCCGGAUCAGCGCUGUCCCCACAACAGc -3' miRNA: 3'- -GGCCUAGUCGCGACAGGGGUGUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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