Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4325 | 3' | -55.4 | NC_001720.1 | + | 20798 | 0.66 | 0.664825 |
Target: 5'- gGCUucGCCcugcugugcggUCC-AUACGGGcCAGCUGCg -3' miRNA: 3'- gCGA--UGG-----------AGGuUGUGCCU-GUCGACGg -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 31213 | 0.66 | 0.661482 |
Target: 5'- aGCUACUUCCuGCAgcagaucaauaccaUGGGCAuGCUGaCUa -3' miRNA: 3'- gCGAUGGAGGuUGU--------------GCCUGU-CGAC-GG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 30347 | 0.66 | 0.631321 |
Target: 5'- cCGCUGCaacaacgaguuCUCCGcuCGCGaGcuGCAGCUGCUg -3' miRNA: 3'- -GCGAUG-----------GAGGUu-GUGC-C--UGUCGACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 24581 | 0.66 | 0.631321 |
Target: 5'- aGCgUACCUCCAAguguucgGCGGGCAcGCggcgGUCg -3' miRNA: 3'- gCG-AUGGAGGUUg------UGCCUGU-CGa---CGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 14066 | 0.66 | 0.620141 |
Target: 5'- gGCUAUCagagCCGauguggACGCGGAgGGUgaUGCCa -3' miRNA: 3'- gCGAUGGa---GGU------UGUGCCUgUCG--ACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 26592 | 0.66 | 0.620141 |
Target: 5'- gGCUcggaGCCggcgguUCCGAgGCGGACGGCgagGUg -3' miRNA: 3'- gCGA----UGG------AGGUUgUGCCUGUCGa--CGg -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 7266 | 0.66 | 0.620141 |
Target: 5'- gCGCaGCCUCUAugGCGauaccGACAGcCUGUUc -3' miRNA: 3'- -GCGaUGGAGGUugUGC-----CUGUC-GACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 18029 | 0.66 | 0.608971 |
Target: 5'- gGCgggGCCggcgCCGGCgccgcgggugcgGCGGGCGGCUcuccggcaGCCa -3' miRNA: 3'- gCGa--UGGa---GGUUG------------UGCCUGUCGA--------CGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 3821 | 0.67 | 0.586703 |
Target: 5'- uGCUAa-UCCAcuGCGCGGACcucucGCUcGCCa -3' miRNA: 3'- gCGAUggAGGU--UGUGCCUGu----CGA-CGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 9441 | 0.67 | 0.575624 |
Target: 5'- gGCaACCUC--GCcCGGAUGGCUGUCu -3' miRNA: 3'- gCGaUGGAGguUGuGCCUGUCGACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 11098 | 0.67 | 0.564592 |
Target: 5'- gCGC-GCUUCgGACGCGGugAuggcuCUGCCg -3' miRNA: 3'- -GCGaUGGAGgUUGUGCCugUc----GACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 22706 | 0.68 | 0.510433 |
Target: 5'- gGCU-CCagaCCcGCGCGGcuguagauaACGGCUGCCa -3' miRNA: 3'- gCGAuGGa--GGuUGUGCC---------UGUCGACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 30767 | 0.68 | 0.499851 |
Target: 5'- aGCauUACCgUCAAUGCGGACGGCgcGCUg -3' miRNA: 3'- gCG--AUGGaGGUUGUGCCUGUCGa-CGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 883 | 0.7 | 0.428821 |
Target: 5'- uGCagUGCCUCCAguuagggcuacGCACGGA--GCUGCa -3' miRNA: 3'- gCG--AUGGAGGU-----------UGUGCCUguCGACGg -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 22393 | 0.79 | 0.115299 |
Target: 5'- gCGCUuuACC-CCAACGCGGACaAGC-GCCg -3' miRNA: 3'- -GCGA--UGGaGGUUGUGCCUG-UCGaCGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 10291 | 0.83 | 0.052488 |
Target: 5'- aCGCgggGCCUCCccuCACGGACAGCgUGCUg -3' miRNA: 3'- -GCGa--UGGAGGuu-GUGCCUGUCG-ACGG- -5' |
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4325 | 3' | -55.4 | NC_001720.1 | + | 6342 | 1.12 | 0.000404 |
Target: 5'- uCGCUACCUCCAACACGGACAGCUGCCg -3' miRNA: 3'- -GCGAUGGAGGUUGUGCCUGUCGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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