Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4325 | 5' | -58.8 | NC_001720.1 | + | 30555 | 0.67 | 0.393432 |
Target: 5'- cGGGCAAGaUGGCCCg----CGcGGGUCc -3' miRNA: 3'- aCCCGUUC-GUCGGGuaguaGC-CCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 9936 | 0.67 | 0.37543 |
Target: 5'- cUGGGCGGGCuuaaAGUCgGggagggagCGuUCGGGGUCu -3' miRNA: 3'- -ACCCGUUCG----UCGGgUa-------GU-AGCCCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 24140 | 0.67 | 0.36664 |
Target: 5'- cGGGUucuGGGUcgGGUUCGUCAUCGcuaGGGUCg -3' miRNA: 3'- aCCCG---UUCG--UCGGGUAGUAGC---CCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 24560 | 0.67 | 0.36664 |
Target: 5'- cGGGCAcGCGGCg----GUCGGGGUUa -3' miRNA: 3'- aCCCGUuCGUCGgguagUAGCCCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 34098 | 0.67 | 0.357994 |
Target: 5'- cGGuGCu-GCAGCaCCGUUAUCGGGa-- -3' miRNA: 3'- aCC-CGuuCGUCG-GGUAGUAGCCCcag -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 28961 | 0.67 | 0.357994 |
Target: 5'- aGGcGCGAcGguGgCCGUgaccgagauacCGUCGGGGUCa -3' miRNA: 3'- aCC-CGUU-CguCgGGUA-----------GUAGCCCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 11052 | 0.68 | 0.33292 |
Target: 5'- gGGGCAGGCggucuacgaaGGCCCGcacggCA-CGGGGcCu -3' miRNA: 3'- aCCCGUUCG----------UCGGGUa----GUaGCCCCaG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 11491 | 0.68 | 0.309158 |
Target: 5'- gGcGGCGAGCAGUUCggCGUCGGcGG-Ca -3' miRNA: 3'- aC-CCGUUCGUCGGGuaGUAGCC-CCaG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 15863 | 0.68 | 0.309158 |
Target: 5'- -cGGCGucacCAGcCCCGUCA-CGGGGUCc -3' miRNA: 3'- acCCGUuc--GUC-GGGUAGUaGCCCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 7477 | 0.68 | 0.30153 |
Target: 5'- cGGGUAuauaAG-GGCCCGUCG-CGaGGGUCg -3' miRNA: 3'- aCCCGU----UCgUCGGGUAGUaGC-CCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 29151 | 0.76 | 0.090878 |
Target: 5'- gGGGagAAGC-GCUgAUCGUCGGGGUCg -3' miRNA: 3'- aCCCg-UUCGuCGGgUAGUAGCCCCAG- -5' |
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4325 | 5' | -58.8 | NC_001720.1 | + | 6305 | 1.1 | 0.00023 |
Target: 5'- aUGGGCAAGCAGCCCAUCAUCGGGGUCa -3' miRNA: 3'- -ACCCGUUCGUCGGGUAGUAGCCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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