miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4326 3' -54.7 NC_001720.1 + 6514 1.1 0.000668
Target:  5'- gGGACUUCUACCAGCGCGUCACCAGCUg -3'
miRNA:   3'- -CCUGAAGAUGGUCGCGCAGUGGUCGA- -5'
4326 3' -54.7 NC_001720.1 + 9487 0.66 0.6659
Target:  5'- aGGGCUUugagacggucgucCUGCCgugcuaagcguucGGCGCGggagACCAGCUg -3'
miRNA:   3'- -CCUGAA-------------GAUGG-------------UCGCGCag--UGGUCGA- -5'
4326 3' -54.7 NC_001720.1 + 10889 0.65 0.698234
Target:  5'- cGACUUCUACCGcaugcucugaccgcGCGCGaCGCgGGaCUc -3'
miRNA:   3'- cCUGAAGAUGGU--------------CGCGCaGUGgUC-GA- -5'
4326 3' -54.7 NC_001720.1 + 11140 0.7 0.45007
Target:  5'- aGGGCgcagGCCGGUGCGgucgguuaccCGCCGGCUg -3'
miRNA:   3'- -CCUGaagaUGGUCGCGCa---------GUGGUCGA- -5'
4326 3' -54.7 NC_001720.1 + 31093 0.66 0.644548
Target:  5'- gGGACUUUgcaacugUACCaAGCGCc-CACUAGCa -3'
miRNA:   3'- -CCUGAAG-------AUGG-UCGCGcaGUGGUCGa -5'
4326 3' -54.7 NC_001720.1 + 38569 0.66 0.668143
Target:  5'- uGGACUUUgACCca-GCGUCACUAgGCg -3'
miRNA:   3'- -CCUGAAGaUGGucgCGCAGUGGU-CGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.