Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4327 | 3' | -58.2 | NC_001720.1 | + | 10791 | 0.66 | 0.501369 |
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Target: 5'- aACGACUC-UCCCAugagucccgCGUCGcGCGCGg -3' miRNA: 3'- -UGCUGGGcAGGGUua-------GCAGCcCGUGC- -5' |
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| 4327 | 3' | -58.2 | NC_001720.1 | + | 21942 | 0.67 | 0.461273 |
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Target: 5'- uACGACUCGUUCCAAggaacUCaGGCACa -3' miRNA: 3'- -UGCUGGGCAGGGUUagc--AGcCCGUGc -5' |
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| 4327 | 3' | -58.2 | NC_001720.1 | + | 20843 | 0.68 | 0.432341 |
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Target: 5'- gAUGGCgUCGuUCCCGAUCaG-CGGGUACGg -3' miRNA: 3'- -UGCUG-GGC-AGGGUUAG-CaGCCCGUGC- -5' |
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| 4327 | 3' | -58.2 | NC_001720.1 | + | 42457 | 0.68 | 0.404495 |
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Target: 5'- -gGAauuauaCCGaCCCAAUCauaGUCGGGUACGa -3' miRNA: 3'- ugCUg-----GGCaGGGUUAG---CAGCCCGUGC- -5' |
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| 4327 | 3' | -58.2 | NC_001720.1 | + | 19474 | 0.7 | 0.290495 |
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Target: 5'- --cGCCCGcCCCGugccCGUCGGGCAgGa -3' miRNA: 3'- ugcUGGGCaGGGUua--GCAGCCCGUgC- -5' |
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| 4327 | 3' | -58.2 | NC_001720.1 | + | 6689 | 1.08 | 0.000526 |
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Target: 5'- uACGACCCGUCCCAAUCGUCGGGCACGa -3' miRNA: 3'- -UGCUGGGCAGGGUUAGCAGCCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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