Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4328 | 3' | -53.4 | NC_001720.1 | + | 7138 | 0.66 | 0.7523 |
Target: 5'- gCCUCUAcugGGCcugcGAUugCGACaucaagugcaaAGCCUGc -3' miRNA: 3'- -GGAGAUa--CCG----CUAugGCUG-----------UCGGACa -5' |
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4328 | 3' | -53.4 | NC_001720.1 | + | 15487 | 0.67 | 0.719841 |
Target: 5'- gUCUCUGUGGCGGccGuuGACGGgucuaaguCCUGa -3' miRNA: 3'- -GGAGAUACCGCUa-UggCUGUC--------GGACa -5' |
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4328 | 3' | -53.4 | NC_001720.1 | + | 25499 | 0.67 | 0.70882 |
Target: 5'- gCCUUgccgAUGGCGGUcggguugggcAgCGAguaCAGCCUGUa -3' miRNA: 3'- -GGAGa---UACCGCUA----------UgGCU---GUCGGACA- -5' |
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4328 | 3' | -53.4 | NC_001720.1 | + | 17489 | 0.68 | 0.615402 |
Target: 5'- gUCUCUGcugcuccuucagauUGGCGAUGCCcacGGCGguGCCUGc -3' miRNA: 3'- -GGAGAU--------------ACCGCUAUGG---CUGU--CGGACa -5' |
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4328 | 3' | -53.4 | NC_001720.1 | + | 11226 | 0.69 | 0.551452 |
Target: 5'- cCCUCUGcagccGGCGGguaACCGACcgcaccGGCCUGc -3' miRNA: 3'- -GGAGAUa----CCGCUa--UGGCUG------UCGGACa -5' |
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4328 | 3' | -53.4 | NC_001720.1 | + | 7261 | 1.09 | 0.001208 |
Target: 5'- gCCUCUAUGGCGAUACCGACAGCCUGUu -3' miRNA: 3'- -GGAGAUACCGCUAUGGCUGUCGGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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