Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4330 | 5' | -48.1 | NC_001720.1 | + | 17181 | 0.72 | 0.732924 |
Target: 5'- --aCCG-GCCGUUUCCGa--GGCGGCu -3' miRNA: 3'- guaGGCuUGGUAAAGGCgauUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 17354 | 0.66 | 0.960148 |
Target: 5'- -cUCUGAuaccuAUCAUUgcggCCGCUAu-CGGCg -3' miRNA: 3'- guAGGCU-----UGGUAAa---GGCGAUuuGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 33866 | 0.66 | 0.95764 |
Target: 5'- ----aGAGCCAUaUCCGCUGcuucaaagcuuuauuGACGGg -3' miRNA: 3'- guaggCUUGGUAaAGGCGAU---------------UUGCCg -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 8360 | 0.67 | 0.935937 |
Target: 5'- gGUCCGAGCaCGUUgggguagcaucgUCCGCcgcgGAGCgcuuGGCg -3' miRNA: 3'- gUAGGCUUG-GUAA------------AGGCGa---UUUG----CCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 18964 | 0.67 | 0.935937 |
Target: 5'- -cUCUGAACCAga-CCGCgu-ACuGGCu -3' miRNA: 3'- guAGGCUUGGUaaaGGCGauuUG-CCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 11193 | 0.68 | 0.917695 |
Target: 5'- -cUCCuGAcagACCcgUUCCuGCUAaggaAACGGCg -3' miRNA: 3'- guAGG-CU---UGGuaAAGG-CGAU----UUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 4200 | 0.68 | 0.910988 |
Target: 5'- gGUCCGAACCGU-UCC-UUGAG-GGCu -3' miRNA: 3'- gUAGGCUUGGUAaAGGcGAUUUgCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 12611 | 0.71 | 0.807988 |
Target: 5'- gGUCCGAGCCGgcUUUCaCGC----CGGCu -3' miRNA: 3'- gUAGGCUUGGU--AAAG-GCGauuuGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 14849 | 0.71 | 0.797787 |
Target: 5'- --aCCGGGCU-UUUUCGCUAGucuGCGGCc -3' miRNA: 3'- guaGGCUUGGuAAAGGCGAUU---UGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 40015 | 0.71 | 0.766058 |
Target: 5'- aGUCCGGuACCAUUUuccaUCGCgaaAAugGGCa -3' miRNA: 3'- gUAGGCU-UGGUAAA----GGCGa--UUugCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 30010 | 0.66 | 0.964098 |
Target: 5'- gGUCCcgcuGACCAcg-CCaccugcagcaGCUAAACGGCa -3' miRNA: 3'- gUAGGc---UUGGUaaaGG----------CGAUUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 31196 | 0.67 | 0.93017 |
Target: 5'- uCGUcCCGGauguaaaugcgGCCAcg-CUGCUAGugGGCg -3' miRNA: 3'- -GUA-GGCU-----------UGGUaaaGGCGAUUugCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 18023 | 0.68 | 0.917695 |
Target: 5'- --gCCGGcGCCGgcgCCGCggguGCGGCg -3' miRNA: 3'- guaGGCU-UGGUaaaGGCGauu-UGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 42482 | 0.68 | 0.917695 |
Target: 5'- cCGUagGAACCGg--CCGgUAGAUGGCa -3' miRNA: 3'- -GUAggCUUGGUaaaGGCgAUUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 30706 | 0.71 | 0.801892 |
Target: 5'- cCGUUCGGGuCCAagaacggaggugugaUUUCCGUggGAGCGGCu -3' miRNA: 3'- -GUAGGCUU-GGU---------------AAAGGCGa-UUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 26590 | 0.72 | 0.755151 |
Target: 5'- -cUCgGAGCCGgcggUUCCGaggcGGACGGCg -3' miRNA: 3'- guAGgCUUGGUa---AAGGCga--UUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 10243 | 1.13 | 0.002636 |
Target: 5'- uCAUCCGAACCAUUUCCGCUAAACGGCu -3' miRNA: 3'- -GUAGGCUUGGUAAAGGCGAUUUGCCG- -5' |
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4330 | 5' | -48.1 | NC_001720.1 | + | 31734 | 0.66 | 0.971158 |
Target: 5'- gGUCCGAugGCCAcggugCCGUUc-ACGGUg -3' miRNA: 3'- gUAGGCU--UGGUaaa--GGCGAuuUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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