Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4331 | 5' | -52.5 | NC_001720.1 | + | 18668 | 0.68 | 0.668394 |
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Target: 5'- aAGCuauuuUCAacACCUgGGUGGAGAGCacUGGUCc -3' miRNA: 3'- -UCG-----AGU--UGGAgUCGCUUCUCG--ACUAG- -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 13228 | 0.68 | 0.622042 |
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Target: 5'- cGCUCAugcGCUUCuGaCGGuaccGAGCUGGUCg -3' miRNA: 3'- uCGAGU---UGGAGuC-GCUu---CUCGACUAG- -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 25012 | 0.69 | 0.564393 |
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Target: 5'- cGCUCAagGCCguggaaaAGCGGAGGGCcgcGGUCa -3' miRNA: 3'- uCGAGU--UGGag-----UCGCUUCUCGa--CUAG- -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 12823 | 0.7 | 0.5193 |
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Target: 5'- aAGCUCAAuCCUCAGCu-GGuGUUGGUg -3' miRNA: 3'- -UCGAGUU-GGAGUCGcuUCuCGACUAg -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 16623 | 0.76 | 0.234306 |
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Target: 5'- cAGUUCAACCcugCAGUGAGGAGCggaagGAUUu -3' miRNA: 3'- -UCGAGUUGGa--GUCGCUUCUCGa----CUAG- -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 9609 | 0.8 | 0.140428 |
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Target: 5'- uGCuUCAugCUCAGCGGAGAcccccaGCUGGUCu -3' miRNA: 3'- uCG-AGUugGAGUCGCUUCU------CGACUAG- -5' |
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| 4331 | 5' | -52.5 | NC_001720.1 | + | 10475 | 1.11 | 0.000857 |
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Target: 5'- cAGCUCAACCUCAGCGAAGAGCUGAUCg -3' miRNA: 3'- -UCGAGUUGGAGUCGCUUCUCGACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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