miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4333 3' -48.1 NC_001720.1 + 23639 0.66 0.962518
Target:  5'- aCGUg----GCgGACUUCGuGGACCUCGa -3'
miRNA:   3'- gGCAaauuaCG-UUGAGGC-UCUGGAGC- -5'
4333 3' -48.1 NC_001720.1 + 6824 0.67 0.958486
Target:  5'- uCCGcgu-GUGUAGCUCUGGGAUgUUGc -3'
miRNA:   3'- -GGCaaauUACGUUGAGGCUCUGgAGC- -5'
4333 3' -48.1 NC_001720.1 + 17185 0.68 0.933898
Target:  5'- gCCGUUUccgagGCGGCUCgCGcAGccGCCUCGu -3'
miRNA:   3'- -GGCAAAuua--CGUUGAG-GC-UC--UGGAGC- -5'
4333 3' -48.1 NC_001720.1 + 20292 0.7 0.844167
Target:  5'- gCUGgg----GCGuCUCCGAGGCCUUGa -3'
miRNA:   3'- -GGCaaauuaCGUuGAGGCUCUGGAGC- -5'
4333 3' -48.1 NC_001720.1 + 29567 0.72 0.730982
Target:  5'- uCCGU--AGUGCAGCUggaugcccugcgCCGAGGCgCUCa -3'
miRNA:   3'- -GGCAaaUUACGUUGA------------GGCUCUG-GAGc -5'
4333 3' -48.1 NC_001720.1 + 12035 1.12 0.003229
Target:  5'- aCCGUUUAAUGCAACUCCGAGACCUCGc -3'
miRNA:   3'- -GGCAAAUUACGUUGAGGCUCUGGAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.