Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4333 | 3' | -48.1 | NC_001720.1 | + | 23639 | 0.66 | 0.962518 |
Target: 5'- aCGUg----GCgGACUUCGuGGACCUCGa -3' miRNA: 3'- gGCAaauuaCG-UUGAGGC-UCUGGAGC- -5' |
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4333 | 3' | -48.1 | NC_001720.1 | + | 6824 | 0.67 | 0.958486 |
Target: 5'- uCCGcgu-GUGUAGCUCUGGGAUgUUGc -3' miRNA: 3'- -GGCaaauUACGUUGAGGCUCUGgAGC- -5' |
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4333 | 3' | -48.1 | NC_001720.1 | + | 17185 | 0.68 | 0.933898 |
Target: 5'- gCCGUUUccgagGCGGCUCgCGcAGccGCCUCGu -3' miRNA: 3'- -GGCAAAuua--CGUUGAG-GC-UC--UGGAGC- -5' |
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4333 | 3' | -48.1 | NC_001720.1 | + | 20292 | 0.7 | 0.844167 |
Target: 5'- gCUGgg----GCGuCUCCGAGGCCUUGa -3' miRNA: 3'- -GGCaaauuaCGUuGAGGCUCUGGAGC- -5' |
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4333 | 3' | -48.1 | NC_001720.1 | + | 29567 | 0.72 | 0.730982 |
Target: 5'- uCCGU--AGUGCAGCUggaugcccugcgCCGAGGCgCUCa -3' miRNA: 3'- -GGCAaaUUACGUUGA------------GGCUCUG-GAGc -5' |
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4333 | 3' | -48.1 | NC_001720.1 | + | 12035 | 1.12 | 0.003229 |
Target: 5'- aCCGUUUAAUGCAACUCCGAGACCUCGc -3' miRNA: 3'- -GGCAAAUUACGUUGAGGCUCUGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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