Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 4336 | 3' | -57.8 | NC_001720.1 | + | 21248 | 0.66 | 0.548305 |
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Target: 5'- aCCGgcucccGGGCCU-CCGGAGGUa- -3' miRNA: 3'- gGGCauaua-UCCGGAcGGCCUCCGgc -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 22933 | 0.66 | 0.516599 |
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Target: 5'- gCCGUAgaccacgGGGUCUGaCGaGGGGCCu -3' miRNA: 3'- gGGCAUaua----UCCGGACgGC-CUCCGGc -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 26244 | 0.66 | 0.516599 |
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Target: 5'- cUCCGgugGUAUAGGCU--CCaGGGGCUGg -3' miRNA: 3'- -GGGCa--UAUAUCCGGacGGcCUCCGGC- -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 40695 | 0.66 | 0.516599 |
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Target: 5'- aCCGUGgacuUGUAGGUgccgCUGUCGGuGGUCu -3' miRNA: 3'- gGGCAU----AUAUCCG----GACGGCCuCCGGc -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 31597 | 0.69 | 0.345782 |
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Target: 5'- uCCCGUGUu--GaGCgaGCCGGugaagauAGGCCGg -3' miRNA: 3'- -GGGCAUAuauC-CGgaCGGCC-------UCCGGC- -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 12393 | 0.7 | 0.314663 |
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Target: 5'- aCCGgucgcGUGcGGGCUggcgGCCGG-GGCCGg -3' miRNA: 3'- gGGCa----UAUaUCCGGa---CGGCCuCCGGC- -5' |
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| 4336 | 3' | -57.8 | NC_001720.1 | + | 14517 | 1.1 | 0.000392 |
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Target: 5'- aCCCGUAUAUAGGCCUGCCGGAGGCCGc -3' miRNA: 3'- -GGGCAUAUAUCCGGACGGCCUCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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