Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4336 | 5' | -49.6 | NC_001720.1 | + | 3234 | 0.66 | 0.925638 |
Target: 5'- cUCgCAGCugCAGauguacuggauagucCAGUCGGUAcauCGCa -3' miRNA: 3'- -AG-GUCGugGUU---------------GUCAGCCAUuauGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 28715 | 0.66 | 0.923147 |
Target: 5'- aUCCGGCAa-AACGGagcUCGG-GGUACACc -3' miRNA: 3'- -AGGUCGUggUUGUC---AGCCaUUAUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 12973 | 0.66 | 0.916701 |
Target: 5'- aCgAGCAgCAGCuGUCGGccaucUGCACg -3' miRNA: 3'- aGgUCGUgGUUGuCAGCCauu--AUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 18023 | 0.66 | 0.909942 |
Target: 5'- gCCGGCGCCGGCGccgCGG--GUGCGg -3' miRNA: 3'- aGGUCGUGGUUGUca-GCCauUAUGUg -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 32403 | 0.66 | 0.895496 |
Target: 5'- aCCAGCagcgccGCCAACAGgaagCGGa---GCGCg -3' miRNA: 3'- aGGUCG------UGGUUGUCa---GCCauuaUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 37854 | 0.67 | 0.863033 |
Target: 5'- gUCUAGCGCCAuCuucaGGgcAUGCACg -3' miRNA: 3'- -AGGUCGUGGUuGucagCCauUAUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 31670 | 0.68 | 0.806361 |
Target: 5'- -aCGGCACCGuggccauCGGacCGGUGGUGCAUa -3' miRNA: 3'- agGUCGUGGUu------GUCa-GCCAUUAUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 19106 | 0.69 | 0.764281 |
Target: 5'- cUCCGGCACCAu--G-CGGgcAUGCAg -3' miRNA: 3'- -AGGUCGUGGUuguCaGCCauUAUGUg -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 19792 | 0.69 | 0.764281 |
Target: 5'- aUCUGGCACCAAU-GUUGGUAA-ACAg -3' miRNA: 3'- -AGGUCGUGGUUGuCAGCCAUUaUGUg -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 13212 | 0.71 | 0.661936 |
Target: 5'- -aCGGUACCGAgcUGGUCGGUuguuAUACACc -3' miRNA: 3'- agGUCGUGGUU--GUCAGCCAu---UAUGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 16612 | 0.76 | 0.377027 |
Target: 5'- -aCGGCGCCggUcGUCGGUGAUcaACACg -3' miRNA: 3'- agGUCGUGGuuGuCAGCCAUUA--UGUG- -5' |
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4336 | 5' | -49.6 | NC_001720.1 | + | 14481 | 1.12 | 0.001671 |
Target: 5'- gUCCAGCACCAACAGUCGGUAAUACACa -3' miRNA: 3'- -AGGUCGUGGUUGUCAGCCAUUAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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