Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4348 | 3' | -54.2 | NC_001720.1 | + | 12707 | 0.72 | 0.386296 |
Target: 5'- cCUACGCCGgCUGCuguGaCGGC-GAGCGGa -3' miRNA: 3'- -GGUGCGGUgGACGu--C-GUCGaUUUGCC- -5' |
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4348 | 3' | -54.2 | NC_001720.1 | + | 12648 | 0.67 | 0.637165 |
Target: 5'- gCGCGCgGCCgaGUugaagagGGCGGCUGAGCa- -3' miRNA: 3'- gGUGCGgUGGa-CG-------UCGUCGAUUUGcc -5' |
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4348 | 3' | -54.2 | NC_001720.1 | + | 10276 | 0.74 | 0.282626 |
Target: 5'- gUCGCGCCGCCgcuucgaugucuucGCGGCGGU--GGCGGa -3' miRNA: 3'- -GGUGCGGUGGa-------------CGUCGUCGauUUGCC- -5' |
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4348 | 3' | -54.2 | NC_001720.1 | + | 9669 | 0.72 | 0.380825 |
Target: 5'- gCAUGUCAUaaacgucugacgaugCUGCAGCAGCguGACGGu -3' miRNA: 3'- gGUGCGGUG---------------GACGUCGUCGauUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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